B6Brd;B6N-Tyrc-Brd Smc3tm1a(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:05853
International strain nameB6Brd;B6N-Tyrc-Brd Smc3tm1a(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0070_2_G01
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSmc3tm1a(EUCOMM)Wtsi
Gene/Transgene symbolSmc3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0070_2_G01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6Brd-Tyrc-Brd or C57BL/6NTac
Breeding at archiving centreReceived as frozen sperm. Due to the possible presence of C57BL/6Brd-Tyrc-Brd this strain may produce albino pups if intercrossed.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • abnormal snout morphology / IMPC
  • decreased body length / IMPC
  • decreased body weight / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • decreased lean body mass / IMPC
  • decreased bone mineral content / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • process of degenerative change / IMPC
  • lipid deposition / IMPC
  • developmental and structural abnormality / IMPC
IMPC phenotypes (gene matching)
  • decreased body weight / IMPC
  • decreased lean body mass / IMPC
  • lipid deposition / IMPC
  • developmental and structural abnormality / IMPC
  • process of degenerative change / IMPC
  • decreased bone mineral content / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • abnormal snout morphology / IMPC
  • decreased body length / IMPC
MGI phenotypes (allele matching)
  • decreased body weight / MGI
  • abnormal coat/hair pigmentation / MGI
  • decreased lean body mass / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • decreased total body fat amount / MGI
  • decreased bone mineral content / MGI
  • abnormal craniofacial morphology / MGI
  • increased T cell number / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
MGI phenotypes (gene matching)
  • abnormal craniofacial morphology / MGI
  • decreased body weight / MGI
  • abnormal coat/hair pigmentation / MGI
  • decreased lean body mass / MGI
  • increased T cell number / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • decreased total body fat amount / MGI
  • decreased bone mineral content / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • Smc3 is required for mouse embryonic and adult hematopoiesis.;Wang Tianjiao, Glover Brandi, Hadwiger Gayla, Miller Christopher A, di Martino Orsola, Welch John S, ;2019;Experimental hematology;70;70-84.e6; 30553776

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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