B6Brd;B6N-Tyrc-Brd Ppp5ctm1a(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:05851
International strain nameB6Brd;B6N-Tyrc-Brd Ppp5ctm1a(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0114_3_G12
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPpp5ctm1a(EUCOMM)Wtsi
Gene/Transgene symbolPpp5c
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0114_3_G12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6Brd-Tyrc-Brd or C57BL/6NTac
Breeding at archiving centreReceived as frozen sperm. May be carrying C57BL/6Brd-Tyrc-Brd therefore carries the potential to produce albino offspring if intercrossed.

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal rib morphology / IMPC
  • increased rib number / IMPC
  • decreased body length / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • decreased circulating triglyceride level / IMPC
  • increased lean body mass / IMPC
  • vertebral fusion / IMPC
  • increased lumbar vertebrae number / IMPC
  • increased thoracic vertebrae number / IMPC
  • decreased circulating glucose level / IMPC
  • increased total body fat amount / IMPC
  • neoplasm / IMPC
IMPC phenotypes (gene matching)
  • abnormal rib morphology / IMPC
  • increased rib number / IMPC
  • increased lean body mass / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • vertebral fusion / IMPC
  • neoplasm / IMPC
  • increased lumbar vertebrae number / IMPC
  • decreased body length / IMPC
  • increased thoracic vertebrae number / IMPC
  • decreased circulating glucose level / IMPC
  • decreased circulating triglyceride level / IMPC
  • increased total body fat amount / IMPC
MGI phenotypes (allele matching)
  • abnormal rib morphology / MGI
  • decreased circulating HDL cholesterol level / MGI
  • decreased body length / MGI
  • decreased body weight / MGI
  • abnormal coat/hair pigmentation / MGI
  • decreased circulating triglyceride level / MGI
  • decreased rib number / MGI
  • abnormal bone structure / MGI
  • decreased lumbar vertebrae number / MGI
  • abnormal behavior / MGI
  • decreased circulating cholesterol level / MGI
  • decreased circulating glucose level / MGI
  • decreased mean corpuscular hemoglobin concentration / MGI
  • lymphoid hyperplasia / MGI
  • vertebral transformation / MGI
  • vertebral fusion / MGI
  • caudal vertebral transformation / MGI
  • caudal vertebral fusion / MGI
  • cervical vertebral fusion / MGI
  • decreased thoracic vertebrae number / MGI
  • truncated ribs / MGI
  • increased T cell number / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased respiratory quotient / MGI
  • increased lymphoma incidence / MGI
MGI phenotypes (gene matching)
  • abnormal rib morphology / MGI
  • decreased circulating HDL cholesterol level / MGI
  • abnormal cell morphology / MGI
  • lymphoid hyperplasia / MGI
  • enlarged spleen / MGI
  • decreased body length / MGI
  • decreased body weight / MGI
  • decreased litter size / MGI
  • abnormal coat/hair pigmentation / MGI
  • no abnormal phenotype detected / MGI
  • decreased circulating triglyceride level / MGI
  • no phenotypic analysis / MGI
  • vertebral transformation / MGI
  • decreased rib number / MGI
  • abnormal cell adhesion / MGI
  • abnormal bone structure / MGI
  • abnormal cell cycle checkpoint function / MGI
  • vertebral fusion / MGI
  • caudal vertebral transformation / MGI
  • caudal vertebral fusion / MGI
  • cervical vertebral fusion / MGI
  • decreased lumbar vertebrae number / MGI
  • decreased thoracic vertebrae number / MGI
  • truncated ribs / MGI
  • abnormal behavior / MGI
  • increased T cell number / MGI
  • decreased circulating cholesterol level / MGI
  • decreased circulating glucose level / MGI
  • decreased mean corpuscular hemoglobin concentration / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased respiratory quotient / MGI
  • lethality, incomplete penetrance / MGI
  • decreased fibroblast proliferation / MGI
  • increased lymphoma incidence / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
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MTA will be issued after an order has been submitted.

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