IMPC phenotypes (gene matching)
129S2(Cg)-Dcxtm1.2Ffr/Orl
| Status | Available to order |
| EMMA ID | EM:05808 |
| Citation information | RRID:IMSR_EM:05808 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129S2(Cg)-Dcxtm1.2Ffr/Orl |
| Alternative name | Dcx knockout Sv129Pas |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Dcxtm1.2Ffr |
| Gene/Transgene symbol | Dcx |
Information from provider
| Provider | Fiona Francis |
| Provider affiliation | Inserm UMRS 839 |
| Genetic information | To generate Dcx knock-out mice (Kappeler et al, 2006) a loxP site containing a BamHI restriction site was inserted in the BsaI site upstream of Dcx exon 3. A floxed PGK-neo selection gene was inserted into the XbaI site downstream of exon 3. Mice carrying a floxed Dcx allele, with loxP sites flanking exon 3 and a floxed selection gene, were crossed with cre transgenic mice expressing the cre recombinase early in development (Leneuve et al, 2003). This cross generated (1) knock-out mice (having deleted Dcx exon 3 and the selection gene) and (2) mice deleted for the selection gene but still carrying the floxed exon 3 which will further allow the inactivation of Dcx in a spatially and temporally controlled manner. Mice are maintained on pure C57BL/6N and 129/SvPas backgrounds. We would like to cryopreserve knock-out mice on the 129/SvPas background. The Dcx gene is on the X chromosome and hence knock-out males are hemizygotes. Crosses with wild-type 129/SvPas females will produce heterozygous females and wild-type males. |
| Phenotypic information | These constitutive knockout mice have hippocampus lamination defects and are a model of hippocampus dysplasia associated with epilepsy. |
| Breeding history | Backcrossing has been performed for at least 10 generations. For cryoconservation we would use hemizygous males crossed with wild-type females. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129S2/SvPas males |
| Breeding at archiving centre | Hemizygous males with wild-type females |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI allele-associated human disease models
Orphanet associated rare diseases, based on orthologous gene matching
- Lissencephaly type 1 due to doublecortin gene mutation / Orphanet_2148
- Subcortical band heterotopia / Orphanet_99796
Literature references
- Branching and nucleokinesis defects in migrating interneurons derived from doublecortin knockout mice.;Kappeler Caroline, Saillour Yoann, Baudoin Jean-Pierre, Tuy Françoise Phan Dinh, Alvarez Chantal, Houbron Christophe, Gaspar Patricia, Hamard Ghislaine, Chelly Jamel, Métin Christine, Francis Fiona, ;2006;Human molecular genetics;15;1387-400; 16571605
- Magnetic resonance imaging and histological studies of corpus callosal and hippocampal abnormalities linked to doublecortin deficiency.;Kappeler Caroline, Dhenain Marc, Phan Dinh Tuy Françoise, Saillour Yoann, Marty Serge, Fallet-Bianco Catherine, Souville Isabelle, Souil Evelyne, Pinard Jean-Marc, Meyer Gundela, Encha-Razavi Ferechté, Volk Andreas, Beldjord Cherif, Chelly Jamel, Francis Fiona, ;2007;The Journal of comparative neurology;500;239-54; 17111359
- Epilepsy in Dcx knockout mice associated with discrete lamination defects and enhanced excitability in the hippocampus.;Nosten-Bertrand Marika, Kappeler Caroline, Dinocourt Céline, Denis Cécile, Germain Johanne, Phan Dinh Tuy Françoise, Verstraeten Soraya, Alvarez Chantal, Métin Christine, Chelly Jamel, Giros Bruno, Miles Richard, Depaulis Antoine, Francis Fiona, ;2008;PloS one;3;e2473; 18575605
- Cellular anatomy, physiology and epileptiform activity in the CA3 region of Dcx knockout mice: a neuronal lamination defect and its consequences.;Bazelot Michael, Simonnet Jean, Dinocourt Céline, Bruel-Jungerman Elodie, Miles Richard, Fricker Desdemona, Francis Fiona, ;2012;The European journal of neuroscience;35;244-56; 22250815