STOCK Ripply3tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05802
Citation informationRRID:IMSR_EM:05802 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameSTOCK Ripply3tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0383_3_E02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolRipply3tm1a(EUCOMM)Wtsi
Gene/Transgene symbolRipply3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0383_3_E02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous males, wild-type C57BL/6J females
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal aorta morphology / MGI
  • abnormal vascular regression / MGI
  • abnormal parathyroid gland morphology / MGI
  • abnormal thymus development / MGI
  • small thymus / MGI
  • cyanosis / MGI
  • abnormal artery morphology / MGI
  • abnormal neural crest cell migration / MGI
  • ectopic thymus / MGI
  • abnormal subclavian artery morphology / MGI
  • abnormal ultimobranchial body morphology / MGI
  • interrupted aortic arch / MGI
  • abnormal pharyngeal pouch morphology / MGI
  • abnormal third pharyngeal arch morphology / MGI
  • abnormal fourth pharyngeal arch morphology / MGI
  • small pharyngeal arch / MGI
  • increased pancreatic beta cell mass / MGI
  • abnormal second pharyngeal arch artery morphology / MGI
  • abnormal pharyngeal arch development / MGI
  • ventricular septal defect / MGI
  • abnormal fetal ductus arteriosus morphology / MGI
  • neonatal lethality, complete penetrance / MGI
  • increased pancreatic beta cell proliferation / MGI
  • absent third pharyngeal arch artery / MGI
  • absent fourth pharyngeal arch artery / MGI
  • increased pancreatic alpha cell mass / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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