C57BL/6NTac-Pex3^{tm1a(EUCOMM)Wtsi}/WtsiOrl

Status	Available to order
EMMA ID	EM:05793
International strain name	C57BL/6NTac-Pex3^{tm1a(EUCOMM)Wtsi}/WtsiOrl
Alternative name	EPD0103_1_H06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Pex3^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Pex3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0103_1_H06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6N Tac

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Zellweger syndrome / Orphanet_912
Neonatal adrenoleukodystrophy / Orphanet_44
Infantile Refsum disease / Orphanet_772

IMPC phenotypes (allele matching)

increased bone mineral density / IMPC
abnormal vertebrae morphology / IMPC
decreased circulating LDL cholesterol level / IMPC
decreased circulating HDL cholesterol level / IMPC
decreased body weight / IMPC
abnormal cornea morphology / IMPC
abnormal eye morphology / IMPC
abnormal tail morphology / IMPC
abnormal eye size / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating serum albumin level / IMPC
corneal vascularization / IMPC
decreased circulating total protein level / IMPC
decreased total body fat amount / IMPC
process of degenerative change / IMPC
inflammation / IMPC
developmental dysplasia / IMPC

IMPC phenotypes (gene matching)

decreased body weight / IMPC
abnormal eye morphology / IMPC
abnormal vertebrae morphology / IMPC
corneal vascularization / IMPC
increased bone mineral density / IMPC
inflammation / IMPC
decreased circulating HDL cholesterol level / IMPC
abnormal tail morphology / IMPC
decreased circulating serum albumin level / IMPC
abnormal eye size / IMPC
abnormal cornea morphology / IMPC
decreased circulating total protein level / IMPC
process of degenerative change / IMPC
developmental dysplasia / IMPC
decreased total body fat amount / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating LDL cholesterol level / IMPC

MGI phenotypes (allele matching)

increased bone mineral density / MGI
decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
abnormal sebaceous gland morphology / MGI
decreased body weight / MGI
abnormal cornea morphology / MGI
decreased caudal vertebrae number / MGI
abnormal eye morphology / MGI
abnormal tail morphology / MGI
abnormal eye size / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating cholesterol level / MGI
decreased circulating serum albumin level / MGI
increased mean corpuscular hemoglobin / MGI
decreased circulating total protein level / MGI
decreased circulating aspartate transaminase level / MGI
abnormal hair follicle bulge morphology / MGI
decreased total body fat amount / MGI
increased circulating fructosamine level / MGI
decreased circulating fructosamine level / MGI
increased sacral vertebrae number / MGI
small sebaceous gland / MGI

MGI phenotypes (gene matching)

increased bone mineral density / MGI
decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
abnormal sebaceous gland morphology / MGI
decreased body weight / MGI
abnormal cornea morphology / MGI
decreased caudal vertebrae number / MGI
abnormal eye morphology / MGI
abnormal tail morphology / MGI
abnormal eye size / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating cholesterol level / MGI
decreased circulating serum albumin level / MGI
increased mean corpuscular hemoglobin / MGI
decreased circulating total protein level / MGI
decreased circulating aspartate transaminase level / MGI
abnormal hair follicle bulge morphology / MGI
decreased total body fat amount / MGI
increased circulating fructosamine level / MGI
decreased circulating fructosamine level / MGI
increased sacral vertebrae number / MGI
small sebaceous gland / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6NTac-Pex3tm1a(EUCOMM)Wtsi/WtsiOrl