C57BL/6N-Agltm1a(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:05784
International strain nameC57BL/6N-Agltm1a(EUCOMM)Wtsi/H
Alternative nameEPD0371_2_E12
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAgltm1a(EUCOMM)Wtsi
Gene/Transgene symbolAgl
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0371_2_E12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6NTac
Breeding at archiving centreChimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

    • Glycogen storage disease due to glycogen debranching enzyme deficiency / Orphanet_366
IMPC phenotypes (gene matching)
  • increased circulating creatine kinase level / IMPC
  • increased circulating amylase level / IMPC
  • increased kidney weight / IMPC
  • cataract / IMPC
  • increased lean body mass / IMPC
  • increased circulating cholesterol level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased bone mineral content / IMPC
  • increased fasting circulating glucose level / IMPC
  • decreased bone mineral density / IMPC
  • impaired glucose tolerance / IMPC
  • increased heart weight / IMPC
  • increased circulating alanine transaminase level / IMPC
  • abnormal gait / IMPC
  • decreased total body fat amount / IMPC
  • tremors / IMPC
  • increased circulating bilirubin level / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • increased circulating iron level / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • decreased circulating glucose level / IMPC
MGI phenotypes (allele matching)
  • hypoglycemia / MGI
  • enlarged liver / MGI
  • abnormal hepatocyte morphology / MGI
  • muscle weakness / MGI
  • hypoactivity / MGI
  • premature death / MGI
  • increased circulating alanine transaminase level / MGI
  • increased circulating alkaline phosphatase level / MGI
  • liver fibrosis / MGI
  • increased circulating aspartate transaminase level / MGI
  • decreased grip strength / MGI
  • increased circulating creatine kinase level / MGI
  • increased liver glycogen level / MGI
  • increased skeletal muscle glycogen level / MGI
  • increased cardiac muscle glycogen level / MGI
MGI phenotypes (gene matching)
  • kyphosis / MGI
  • hypoglycemia / MGI
  • abnormal liver morphology / MGI
  • enlarged liver / MGI
  • abnormal hepatocyte morphology / MGI
  • muscle weakness / MGI
  • hypoactivity / MGI
  • impaired coordination / MGI
  • abnormal gait / MGI
  • premature death / MGI
  • increased circulating alanine transaminase level / MGI
  • increased circulating alkaline phosphatase level / MGI
  • liver fibrosis / MGI
  • increased circulating aspartate transaminase level / MGI
  • increased glycogen level / MGI
  • decreased circulating glucose level / MGI
  • increased pulmonary respiratory rate / MGI
  • decreased grip strength / MGI
  • increased circulating creatine kinase level / MGI
  • increased liver glycogen level / MGI
  • increased skeletal muscle glycogen level / MGI
  • impaired exercise endurance / MGI
  • decreased fasted circulating glucose level / MGI
  • increased cardiac muscle glycogen level / MGI
  • increased brain glycogen level / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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