MGI phenotypes (allele matching)
- immune system phenotype / MGI
C57BL/6-Gzmbtm1Asmv/Orl
| Status | Available to order |
| EMMA ID | EM:05732 |
| International strain name | C57BL/6-Gzmbtm1Asmv/Orl |
| Alternative name | Gzmb-Tom |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gzmbtm1Asmv |
| Gene/Transgene symbol | Gzmb |
Information from provider
| Provider | Anne-Marie Schmitt-Verhulst |
| Provider affiliation | Centre D'immunologie Marseille-Luminy |
| Additional owner | Pierre Mouchacca, Claude Boyer, Anne-Marie Schmitt-Verhulst CIML, Marseille |
| Genetic information | Insertion into Gzmb gene of a construct composed of cDNA Gzmb without stop codon-linker-cDNA TdTomato. |
| Phenotypic information | In the Gzmb-Tom strain a red fluorescent chimeric protein granzyme b-tdTomato is expressed in place of endogenous granzyme b, allowing for detection of effector cytolytic T cells and NK cells by either FACS or fluorescence microscopy. Red fluorescence is detected in cytolytic granules, the dynamics of which can be observed on live cells in vitro or in vivo. |
| Breeding history | Generated from a C57BL/6 ES cell clone; the mice were backcrossed on C57BL/6 for 3 generations before being inter-crossed. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J, wild-type C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal immune system physiology / MGI
- decreased tumor growth/size / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- decreased cytotoxic T cell cytolysis / MGI
- immune system phenotype / MGI
- abnormal enzyme/coenzyme activity / MGI
- decreased T cell apoptosis / MGI
- increased sensitivity to induced morbidity/mortality / MGI
- abnormal NK cell physiology / MGI
Literature references
- Visualization of cytolytic T cell differentiation and granule exocytosis with T cells from mice expressing active fluorescent granzyme B.;Mouchacca Pierre, Schmitt-Verhulst Anne-Marie, Boyer Claude, ;2013;PloS one;8;e67239; 23840635