B6Brd;B6N-Tyr^c-Brd Hdac3^{tm1a(EUCOMM)Wtsi}/WtsiBiat

Status	Available to order
EMMA ID	EM:05711
International strain name	B6Brd;B6N-Tyr^c-Brd Hdac3^{tm1a(EUCOMM)Wtsi}/WtsiBiat
Alternative name	EPD0028_3_D01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Hdac3^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Hdac3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0028_3_D01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating LDL cholesterol level / IMPC
decreased hematocrit / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

decreased hemoglobin content / IMPC
increased circulating LDL cholesterol level / IMPC
decreased hematocrit / IMPC
decreased erythrocyte cell number / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (gene matching)

abnormal gastrulation / MGI
no phenotypic analysis / MGI
embryonic lethality, complete penetrance / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
Histone deacetylase 3 promotes liver regeneration and liver cancer cells proliferation through signal transducer and activator of transcription 3 signaling pathway.;Lu Xu-Feng, Cao Xiao-Yue, Zhu Yong-Jie, Wu Zhen-Ru, Zhuang Xiang, Shao Ming-Yang, Xu Qing, Zhou Yong-Jie, Ji Hong-Jie, Lu Qing-Richard, Shi Yu-Jun, Zeng Yong, Bu Hong, ;2018;Cell death & disease;9;398; 29540666
HDAC3 Deficiency Promotes Liver Cancer through a Defect in H3K9ac/H3K9me3 Transition.;Ji Hongjie, Zhou Yongjie, Zhuang Xiang, Zhu Yongjie, Wu Zhenru, Lu Yannrong, Li Shengfu, Zeng Yong, Lu Qing R, Huo Yanying, Shi Yujun, Bu Hong, ;2019;Cancer research;79;3676-3688; 31097476

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Hdac3tm1a(EUCOMM)Wtsi/WtsiBiat