C57BL/6NTac-Ppp3catm2e(EUCOMM)Wtsi/WtsiBiat

Status

Available to order

EMMA IDEM:05704
International strain nameC57BL/6NTac-Ppp3catm2e(EUCOMM)Wtsi/WtsiBiat
Alternative nameEPD0200_3_B01
Strain typeTargeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbolPpp3catm2e(EUCOMM)Wtsi
Gene/Transgene symbolPpp3ca
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0200_3_B01. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • increased body weight / IMPC
  • abnormal tail movements / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • decreased mean corpuscular volume / IMPC
  • increased blood urea nitrogen level / IMPC
  • decreased circulating amylase level / IMPC
  • increased total body fat amount / IMPC
  • decreased circulating magnesium level / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating magnesium level / IMPC
  • increased body weight / IMPC
  • increased blood urea nitrogen level / IMPC
  • decreased circulating amylase level / IMPC
  • decreased mean corpuscular volume / IMPC
  • abnormal tail movements / IMPC
  • increased total body fat amount / IMPC
  • abnormal coat/hair pigmentation / IMPC
MGI phenotypes (gene matching)
  • decreased circulating calcium level / MGI
  • decreased body weight / MGI
  • premature death / MGI
  • abnormal hippocampal mossy fiber morphology / MGI
  • abnormal urine osmolality / MGI
  • decreased urine osmolality / MGI
  • decreased T cell proliferation / MGI
  • increased circulating creatinine level / MGI
  • decreased circulating glucose level / MGI
  • increased blood urea nitrogen level / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased interleukin-2 secretion / MGI
  • decreased interleukin-4 secretion / MGI
  • increased skeletal muscle fiber diameter / MGI
  • decreased skeletal muscle fiber number / MGI
  • abnormal skeletal muscle fiber type ratio / MGI
  • decreased urine creatinine level / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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