STOCK Cdk5rap2^{tm1a(EUCOMM)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:05702
International strain name	STOCK Cdk5rap2^{tm1a(EUCOMM)Wtsi}/WtsiIeg
Alternative name	EPD0381_4_F01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cdk5rap2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cdk5rap2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0381_4_F01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive primary microcephaly / Orphanet_2512

IMPC phenotypes (allele matching)

decreased leukocyte cell number / IMPC
abnormal head morphology / IMPC
abnormal cranium morphology / IMPC
decreased body weight / IMPC
increased mean corpuscular volume / IMPC
decreased erythrocyte cell number / IMPC
decreased lean body mass / IMPC
increased mean corpuscular hemoglobin / IMPC
decreased bone mineral content / IMPC
hypoplasia / IMPC
hyperplasia / IMPC
extramedullary hemopoiesis / IMPC

IMPC phenotypes (gene matching)

decreased body weight / IMPC
decreased lean body mass / IMPC
increased mean corpuscular volume / IMPC
abnormal head morphology / IMPC
hyperplasia / IMPC
hypoplasia / IMPC
decreased bone mineral content / IMPC
extramedullary hemopoiesis / IMPC
decreased leukocyte cell number / IMPC
decreased erythrocyte cell number / IMPC
increased mean corpuscular hemoglobin / IMPC
abnormal cranium morphology / IMPC

MGI phenotypes (allele matching)

decreased leukocyte cell number / MGI
abnormal head morphology / MGI
abnormal cranium morphology / MGI
decreased body weight / MGI
increased mean corpuscular volume / MGI
decreased lean body mass / MGI
increased mean corpuscular hemoglobin / MGI
decreased bone mineral content / MGI

MGI phenotypes (gene matching)

kyphosis / MGI
decreased leukocyte cell number / MGI
abnormal erythropoiesis / MGI
macrocytosis / MGI
abnormal head morphology / MGI
microcephaly / MGI
abnormal cranium morphology / MGI
decreased brain size / MGI
abnormal cerebral cortex morphology / MGI
abnormal spermatogenesis / MGI
decreased body weight / MGI
decreased body size / MGI
anemia / MGI
reduced fertility / MGI
male infertility / MGI
postnatal lethality / MGI
premature death / MGI
decreased germ cell number / MGI
abnormal erythrocyte morphology / MGI
increased mean corpuscular volume / MGI
anisocytosis / MGI
small olfactory bulb / MGI
macrocytic anemia / MGI
decreased erythrocyte cell number / MGI
abnormal cell nucleus morphology / MGI
increased neuron apoptosis / MGI
abnormal forebrain development / MGI
loss of cortex neurons / MGI
abnormal plasma membrane morphology / MGI
decreased lean body mass / MGI
abnormal mitosis / MGI
abnormal embryonic/fetal subventricular zone morphology / MGI
premature hair loss / MGI
cellular phenotype / MGI
decreased oocyte number / MGI
increased mean corpuscular hemoglobin / MGI
thin cerebral cortex / MGI
early cellular replicative senescence / MGI
small hippocampus / MGI
reticulocytopenia / MGI
abnormal mitotic spindle morphology / MGI
eye opacity / MGI
abnormal neuron proliferation / MGI
decreased bone mineral content / MGI
enlarged brain ventricles / MGI
increased ovary adenoma incidence / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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