B6NTac;B6N-A^tm1Brd Barx2^{tm1a(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:05650
Citation information	RRID:IMSR_EM:05650 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6NTac;B6N-A^tm1Brd Barx2^{tm1a(EUCOMM)Hmgu}/H
Alternative name	HEPD0634_2_F07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Barx2^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Barx2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0634_2_F07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC
absent vibrissae / IMPC
abnormal gait / IMPC
abnormal coat/hair pigmentation / IMPC
increased circulating bilirubin level / IMPC
increased circulating amylase level / IMPC
abnormal eye morphology / IMPC
increased circulating creatine kinase level / IMPC
increased circulating aspartate transaminase level / IMPC
abnormal tail morphology / IMPC
abnormal vertebrae morphology / IMPC

MGI phenotypes (gene matching)

alopecia / MGI
short hair / MGI
abnormal hair cycle / MGI
enlarged sebaceous gland / MGI
abnormal muscle regeneration / MGI
abnormal skeletal muscle morphology / MGI
thin diaphragm muscle / MGI
decreased body weight / MGI
short vibrissae / MGI
eyelids open at birth / MGI
abnormal gait / MGI
disheveled coat / MGI
abnormal motor capabilities/coordination/movement / MGI
abnormal skin pigmentation / MGI
abnormal skeletal muscle fiber morphology / MGI
abnormal muscle fiber morphology / MGI
abnormal spine curvature / MGI
decreased skeletal muscle mass / MGI
slow postnatal weight gain / MGI
decreased skeletal muscle fiber diameter / MGI
increased variability of skeletal muscle fiber size / MGI
skeletal muscle fiber atrophy / MGI
skeletal muscle fibrosis / MGI
decreased skeletal muscle weight / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6NTac;B6N-Atm1Brd Barx2tm1a(EUCOMM)Hmgu/H