IMPC phenotypes (gene matching)
- decreased cardiac muscle contractility / IMPC
B6(Cg)-Clec9atm1.1Crs/H
| Status | Available to order |
| EMMA ID | EM:05605 |
| International strain name | B6(Cg)-Clec9atm1.1Crs/H |
| Alternative name | DNGR-1 KO C57BL/6J |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Clec9atm1.1Crs |
| Gene/Transgene symbol | Clec9a |
Information from provider
| Provider | Neil Rogers |
| Provider affiliation | Translational Oncology, Cancer Research UK |
| Additional owner | Cancer Research UK (CRUK), London, UK |
| Genetic information | Homozygous for knock-out mutation (a knock-in of a farnesylated form of EGFP inserted in frame with the two first amino acids of the gene locus). Half of the exon 1 and the entire exon 2 were eliminated and transcription following the EGFP is terminated by a strong polyadenylation signal. A loxP flanked neomycin cassette was included downstream of EGFP for targeting purposes and was subsequently deleted by crossing to a PGK-Cre mouse. |
| Phenotypic information | The CD8+ve family of dendritic cells lack expression of DNGR-1 (CLEC9A) and are impaired in the cross-priming of T-cells. |
| Breeding history | Knock-out generated in the (129S6 x C57BL/6)F1 derived ES cell line S6B6 (equivalent to B6 N1). After crossing 2x to C57BL/6J (Charles River), they were crossed 1x to C57BL/6J PGK-Cre to delete the NeoR gene. The strain is now Cre negative after being crossed a further 7x to C57BL/6J 7x to C57BL/6J Charles River. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous C57BL/6J |
| Breeding at archiving centre | Males were archived upon arrival. No breeding was performed at the archiving centre. |
Disease and phenotype information
Literature references
- Identification of a dendritic cell receptor that couples sensing of necrosis to immunity.;Sancho David, Joffre Olivier P, Keller Anna M, Rogers Neil C, Martínez Dolores, Hernanz-Falcón Patricia, Rosewell Ian, Reis e Sousa Caetano, ;2009;Nature;458;899-903; 19219027