(C57BL/6J x B10.Cg-H2k Tg(Cd74-rtTA)#Doi Tg(tetO-Cd74/MCC)#Doi)F1/Ieg
| Status | Available to order |
| EMMA ID | EM:05574 |
| International strain name | (C57BL/6J x B10.Cg-H2k Tg(Cd74-rtTA)#Doi Tg(tetO-Cd74/MCC)#Doi)F1/Ieg |
| Alternative name | Ii-rTAxTIM |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Cd74-rtTA)#Doi, Tg(tetO-Cd74/MCC)#Doi |
| Gene/Transgene symbol | Tg(Cd74-rtTA)#Doi, Tg(tetO-Cd74/MCC)#Doi |
Information from provider
| Provider | Reinhard Obst |
| Provider affiliation | Institute for Immunology, Ludwig-Maximilians-Universität Munich |
| Additional owner | Christophe Benoist, Department of Microbiology and Immunology, Harvard Medical School, Boston MA, USA |
| Genetic information | Tg(Cd74-rtTA)#Doi: The Cd74 promoter, together with the H2-Ea enhancer, drives the expression of an improved reverse tet transactivator, rtTA-S2. Four independent lines were produced. An un-named line showing the lowest liver expression was used. Tg(tetO-Cd74/MCC)#Doi: Seven tetO sequences were combined with the CMV minimal promoter to drive expression, when induced by doxycycline, of a construct composed of the Cd74 cDNA and a segment of moth cytocrome c cDNA encoding amino acid residues 93 to 103, which replace the CLIP region of Cd74 (amino acid residues 88 to 98). |
| Phenotypic information | Both transgenes confer inducibility of the moth cytochrome C antigen presented by H-2Ek following doxycyclin administration, mostly by dendritic cells. |
| Breeding history | Backcrossed more than 10 generations to B10.BR-H2k H2-T18a/SgSnJ. Currently brother x sister matings. C57BL/6 or C57BL/10 best conventional background usable for this line. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous B10.BR-H2k, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Literature references
- Antigen persistence is required throughout the expansion phase of a CD4(+) T cell response.;Obst Reinhard, van Santen Hisse-Martien, Mathis Diane, Benoist Christophe, ;2005;The Journal of experimental medicine;201;1555-65; 15897273
- Number of T reg cells that differentiate does not increase upon encounter of agonist ligand on thymic epithelial cells.;van Santen Hisse-Martien, Benoist Christophe, Mathis Diane, ;2004;The Journal of experimental medicine;200;1221-30; 15534371