STOCK Apc^tm2.1Cip/Orl

Status	Available to order
EMMA ID	EM:05566
International strain name	STOCK Apc^tm2.1Cip/Orl
Alternative name	Apc-lox-exon14
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Apc^tm2.1Cip
Gene/Transgene symbol	Apc

Information from provider

Provider	Sabine Colnot
Provider affiliation	INSERM
Additional owner	Dr M. Giovannini, Center for Neural Tumor Research, House Ear Institute, Los Angeles, CA, USA
Genetic information	Targeting construct is derived from 129 DNA. The loxP sites are located in the 13rd and in the 14th intron, and there is no selection cassette remaining in the floxed allele; cre-mediated recombination between the loxP sites will excise the 14th exon (Apc-delta14 allele) generating a stop codon at AA579.
Phenotypic information	After cre-mediated recombination, Apc protein loses its domain required for beta-catenin degradation. Consequently, it activates a sustained beta-catenin signaling.
Breeding history	4 backcrosses to C57BL/6 background and cryopreserved after the first homozygous mating.
References	Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles.;Leneuve Patricia, Colnot Sabine, Hamard Ghislaine, Francis Fiona, Niwa-Kawakita Michiko, Giovannini Marco, Holzenberger Martin, ;2003;Nucleic acids research;31;e21; 12595570 Colorectal cancers in a new mouse model of familial adenomatous polyposis: influence of genetic and environmental modifiers.;Colnot Sabine, Niwa-Kawakita Michiko, Hamard Ghislaine, Godard Cécile, Le Plenier Servane, Houbron Christophe, Romagnolo Béatrice, Berrebi Dominique, Giovannini Marco, Perret Christine, ;2004;Laboratory investigation; a journal of technical methods and pathology;84;1619-30; 15502862 Liver-targeted disruption of Apc in mice activates beta-catenin signaling and leads to hepatocellular carcinomas.;Colnot S, Decaens T, Niwa-Kawakita M, Godard C, Hamard G, Kahn A, Giovannini M, Perret C, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;17216-21; 15563600 Crypt-restricted proliferation and commitment to the Paneth cell lineage following Apc loss in the mouse intestine.;Andreu Pauline, Colnot Sabine, Godard Cécile, Gad Sophie, Chafey Philippe, Niwa-Kawakita Michiko, Laurent-Puig Pierre, Kahn Axel, Robine Sylvie, Perret Christine, Romagnolo Béatrice, ;2005;Development (Cambridge, England);132;1443-51; 15716339 Apc tumor suppressor gene is the "zonation-keeper" of mouse liver.;Benhamouche Samira, Decaens Thomas, Godard Cécile, Chambrey Régine, Rickman David S, Moinard Christophe, Vasseur-Cognet Mireille, Kuo Calvin J, Kahn Axel, Perret Christine, Colnot Sabine, ;2006;Developmental cell;10;759-70; 16740478 Stabilization of beta-catenin affects mouse embryonic liver growth and hepatoblast fate.;Decaens Thomas, Godard Cécile, de Reyniès Aurélien, Rickman David S, Tronche François, Couty Jean-Pierre, Perret Christine, Colnot Sabine, ;2008;Hepatology (Baltimore, Md.);47;247-58; 18038450 ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription.;Riou Rozenn, Ladli Meriem, Gerbal-Chaloin Sabine, Bossard Pascale, Gougelet Angélique, Godard Cécile, Loesch Robin, Lagoutte Isabelle, Lager Franck, Calderaro Julien, Dos Santos Alexandre, Wang Zhong, Verdier Frédérique, Colnot Sabine, ;2020;eLife;9;; 33084574
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6J, wild-type C57BL/6J

Disease and phenotype information

MGI allele-associated human disease models

hepatocellular carcinoma / DOID:684

Orphanet associated rare diseases, based on orthologous gene matching

Desmoid tumor / Orphanet_873
Cenani-Lenz syndrome / Orphanet_3258
Turcot syndrome with polyposis / Orphanet_99818
Gastric adenocarcinoma and proximal polyposis of the stomach / Orphanet_314022
Gardner syndrome / Orphanet_79665
APC-related attenuated familial adenomatous polyposis / Orphanet_247806

MGI phenotypes (gene matching)

absent mandible / MGI
decreased hematocrit / MGI
increased cell proliferation / MGI
abnormal digestive system morphology / MGI
gastrointestinal hemorrhage / MGI
abnormal foregut morphology / MGI
abnormal crypts of Lieberkuhn morphology / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
abnormal mammary gland development / MGI
mammary gland hyperplasia / MGI
abnormal brain development / MGI
abnormal floor plate morphology / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
dermal cysts / MGI
decreased body weight / MGI
decreased body size / MGI
increased metastatic potential / MGI
abnormal nursing / MGI
hunched posture / MGI
hyperlipidemia / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
anemia / MGI
extended life span / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
decreased embryo size / MGI
incomplete embryo turning / MGI
postnatal growth retardation / MGI
increased mammary adenocarcinoma incidence / MGI
neoplasm / MGI
increased pilomatricoma incidence / MGI
increased tumor incidence / MGI
increased incidence of induced tumors / MGI
increased colon adenoma incidence / MGI
premature death / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
increased intestinal adenoma incidence / MGI
hepatic steatosis / MGI
increased trichoepithelioma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
no phenotypic analysis / MGI
abnormal egg cylinder morphology / MGI
acrania / MGI
colon polyps / MGI
intestinal obstruction / MGI
melena / MGI
rectal hemorrhage / MGI
gastric polyps / MGI
increased hepatocellular carcinoma incidence / MGI
epidermal cyst / MGI
decreased tumor growth/size / MGI
abnormal tumor morphology / MGI
abnormal intestinal goblet cell morphology / MGI
increased tumor growth/size / MGI
increased osteosarcoma incidence / MGI
decreased macrophage cell number / MGI
abnormal embryonic epiblast morphology / MGI
aneuploidy / MGI
abnormal primitive node morphology / MGI
cardia bifida / MGI
increased incidence of tumors by chemical induction / MGI
split notochord / MGI
ovary atrophy / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
uterus atrophy / MGI
decreased T cell number / MGI
increased circulating cholesterol level / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
abnormal cell physiology / MGI
increased apoptosis / MGI
abnormal hindbrain development / MGI
absent male preputial gland / MGI
intestine polyps / MGI
increased liver tumor incidence / MGI
decreased NK cell number / MGI
abnormal anterior visceral endoderm morphology / MGI
embryonic lethality / MGI
abnormal enterocyte proliferation / MGI
adrenal gland hyperplasia / MGI
abnormal mesendoderm development / MGI
increased small intestine adenocarcinoma incidence / MGI
increased large intestine adenocarcinoma incidence / MGI
decreased splenocyte number / MGI
abnormal pregnancy / MGI
absent nipple / MGI
increased prostaglandin level / MGI
embryonic lethality between implantation and placentation / MGI
increased gastrointestinal tumor incidence / MGI
increased mammary gland tumor incidence / MGI
increased skin tumor incidence / MGI
increased stomach tumor incidence / MGI
increased osteoma incidence / MGI
increased desmoid tumor incidence / MGI
gastrointestinal tract polyps / MGI
increased adenoma incidence / MGI
altered tumor pathology / MGI
mortality/aging / MGI
postnatal lethality, complete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
embryonic lethality between implantation and somite formation, incomplete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
embryonic-extraembryonic boundary constriction / MGI
absent forebrain / MGI
rostral-caudal axis duplication / MGI
rostral body truncation / MGI
absent neural folds / MGI
abnormal head development / MGI
decreased intestinal adenoma incidence / MGI
abnormal endocardial heart tube morphology / MGI

Literature references

Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles.;Leneuve Patricia, Colnot Sabine, Hamard Ghislaine, Francis Fiona, Niwa-Kawakita Michiko, Giovannini Marco, Holzenberger Martin, ;2003;Nucleic acids research;31;e21; 12595570
Colorectal cancers in a new mouse model of familial adenomatous polyposis: influence of genetic and environmental modifiers.;Colnot Sabine, Niwa-Kawakita Michiko, Hamard Ghislaine, Godard Cécile, Le Plenier Servane, Houbron Christophe, Romagnolo Béatrice, Berrebi Dominique, Giovannini Marco, Perret Christine, ;2004;Laboratory investigation; a journal of technical methods and pathology;84;1619-30; 15502862
Liver-targeted disruption of Apc in mice activates beta-catenin signaling and leads to hepatocellular carcinomas.;Colnot S, Decaens T, Niwa-Kawakita M, Godard C, Hamard G, Kahn A, Giovannini M, Perret C, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;17216-21; 15563600
Crypt-restricted proliferation and commitment to the Paneth cell lineage following Apc loss in the mouse intestine.;Andreu Pauline, Colnot Sabine, Godard Cécile, Gad Sophie, Chafey Philippe, Niwa-Kawakita Michiko, Laurent-Puig Pierre, Kahn Axel, Robine Sylvie, Perret Christine, Romagnolo Béatrice, ;2005;Development (Cambridge, England);132;1443-51; 15716339
Apc tumor suppressor gene is the "zonation-keeper" of mouse liver.;Benhamouche Samira, Decaens Thomas, Godard Cécile, Chambrey Régine, Rickman David S, Moinard Christophe, Vasseur-Cognet Mireille, Kuo Calvin J, Kahn Axel, Perret Christine, Colnot Sabine, ;2006;Developmental cell;10;759-70; 16740478
Stabilization of beta-catenin affects mouse embryonic liver growth and hepatoblast fate.;Decaens Thomas, Godard Cécile, de Reyniès Aurélien, Rickman David S, Tronche François, Couty Jean-Pierre, Perret Christine, Colnot Sabine, ;2008;Hepatology (Baltimore, Md.);47;247-58; 18038450
ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription.;Riou Rozenn, Ladli Meriem, Gerbal-Chaloin Sabine, Bossard Pascale, Gougelet Angélique, Godard Cécile, Loesch Robin, Lagoutte Isabelle, Lager Franck, Calderaro Julien, Dos Santos Alexandre, Wang Zhong, Verdier Frédérique, Colnot Sabine, ;2020;eLife;9;; 33084574

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

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Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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STOCK Apctm2.1Cip/Orl