IMPC phenotypes (gene matching)
CBA.129S(B6)-Otoatm1Gpr/H
| Status | Available to order |
| EMMA ID | EM:05546 |
| International strain name | CBA.129S(B6)-Otoatm1Gpr/H |
| Alternative name | CBA/OtoaEGFP |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Otoatm1Gpr |
| Gene/Transgene symbol | Otoa |
Information from provider
| Provider | Guy Richardson |
| Provider affiliation | Ancillary Unit, University of Sussex |
| Genetic information | An Otoa null mutant allele was generated by inserting EGFP in frame into exon 2 of Otoa using a targeting construct prepared from a 129/Sv BAC clone. EGFP is expressed under the control of the Otoa promoter. |
| Phenotypic information | In null mutant (OtoaEGFP/EGFP) mice the tectorial membrane is detached at the spiral limbus but remains attached to the outer hair cells, Hensen's stripe is missing and the marginal band appears abnormal. Null mutants show hearing loss but excitation of the outer hair cells and cochlear amplification are almost normal. Many mouse strains do not behave well under anaesthesia and/or suffer from age related hearing loss, especially in the high-frequency basal end of the cochlea. Neither the C57BL/6 nor the CBA strains mice are very suitable for recording intra- and extracellular potentials from cochlear hair cells under anaesthesia, and the C57BL/6 strain suffers from age related hearing loss (due to a mutation in the Cdh23 gene at the Ahl locus). Therefore, if the scientists who work with these mice want to do physiology on anaesthetised animals, it is proposed that they order both strains and use (CBA x C57BL/6)F1 hybrids for experimentation. |
| Breeding history | Original 129/SvEv:C57BL/6J mice have been outbred to CBA to F13. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | heterozygous CBA |
| Breeding at archiving centre | Males were archived upon arrival. No breeding was performed at the archiving centre. |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636