| Status | Available to order |
| EMMA ID | EM:05540 |
| International strain name | B6.129S-Tectbtm1Gpr/H |
| Alternative name | C57Bl6J/Tectbtm1Gpr |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Tectbtm1Gpr |
| Gene/Transgene symbol | Tectb |
| Provider | Guy Richardson |
| Provider affiliation | Ancillary Unit, University of Sussex |
| Genetic information | Exons 1 to 4 of the Tectb gene were deleted and replaced by a neomycin resistance cassette using a targeting construct derived from 129/SvEvBrd DNA. |
| Phenotypic information | Homozygous mutant mice suffer low frequency hearing loss and have normally attached tectorial membranes that lack striated sheet matrix. Hensen's stripe is missing and the marginal band is absent in the apical coil of the cochlea. Sharpness of high frequency tuning is enhanced. Many mouse strains do not behave well under anaesthesia and/or suffer from age related hearing loss, especially in the high-frequency basal end of the cochlea. Neither the C57BL/6 nor the CBA strains mice are very suitable for recording intra- and extracellular potentials from cochlear hair cells under anaesthesia, and the C57BL/6 strain suffers from age related hearing loss (due to a mutation in the Cdh23 gene at the Ahl locus). Therefore, if the scientists who work with these mice want to do physiology on anaesthetised animals, it is proposed that they order both strains and use (CBA x C57BL/6)F1 hybrids for experimentation. |
| Breeding history | Original 129/SvEv:C57BL/6J mice have been outbred to C57BL/6J to F26. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | heterozygous C57BL/6J |
| Breeding at archiving centre | Males were archived upon arrival. No breeding was performed at the archiving centre. |
