B6.129S-Tecta^tm1Gpr/H

Status	Available to order
EMMA ID	EM:05538
International strain name	B6.129S-Tecta^tm1Gpr/H
Alternative name	C57Bl6J/Tectatm1Gpr
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Tecta^tm1Gpr
Gene/Transgene symbol	Tecta

Information from provider

Provider	Guy Richardson
Provider affiliation	Ancillary Unit, University of Sussex
Genetic information	A neomycin resistance cassette was inserted into exon 3 of Tecta using a targeting construct derived from 129/SvEvBrd DNA.
Phenotypic information	Homozygous mutant mice suffer hearing loss and have detached tectorial membranes lacking all non-collagenous matrix. Insertion of the neo cassette results in exon skipping, producing a Tecta transcript in which exons 2 and 4 are joined. While the encoded protein is undetectable in homozygous mutant mice by Western blotting, immunofluorescence analysis shows immunoreactivity in the greater epithelial ridge and in a few small punctate spots in the tectorial membrane. Many mouse strains do not behave well under anaesthesia and/or suffer from age related hearing loss, especially in the high-frequency basal end of the cochlea. Neither the C57BL/6 nor the CBA strains mice are very suitable for recording intra- and extracellular potentials from cochlear hair cells under anaesthesia, and the C57BL/6 strain suffers from age related hearing loss (due to a mutation in the Cdh23 gene at the Ahl locus). Therefore, if the scientists who work with these mice want to do physiology on anaesthetised animals, it is proposed that they order both strains and use (CBA x C57BL/6)F1 hybrids for experimentation.
Breeding history	Original 129/SvEv:C57BL/6J mice have been outbred to C57BL/6J to F34.
References	A targeted deletion in alpha-tectorin reveals that the tectorial membrane is required for the gain and timing of cochlear feedback.;Legan P K, Lukashkina V A, Goodyear R J, Kössi M, Russell I J, Richardson G P, ;2000;Neuron;28;273-85; 11087000
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6J
Breeding at archiving centre	Males were archived upon import to EMMA node. No breeding was performed.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636
Autosomal dominant non-syndromic sensorineural deafness type DFNA / Orphanet_90635

MGI phenotypes (allele matching)

abnormal hearing physiology / MGI
abnormal otolithic membrane morphology / MGI
enlarged otoliths / MGI
decreased otolith number / MGI
abnormal tectorial membrane morphology / MGI
detached tectorial membrane / MGI
abnormal cochlear microphonics / MGI
decreased cochlear nerve compound action potential / MGI
abnormal hair cell mechanoelectric transduction / MGI
abnormal cochlear outer hair cell physiology / MGI
abnormal distortion product otoacoustic emission / MGI
absent distortion product otoacoustic emissions / MGI
behavior/neurological phenotype / MGI
abnormal tectorial membrane striated-sheet matrix morphology / MGI

MGI phenotypes (gene matching)

audiogenic seizures / MGI
abnormal hearing physiology / MGI
abnormal otolithic membrane morphology / MGI
no phenotypic analysis / MGI
enlarged otoliths / MGI
decreased otolith number / MGI
abnormal tectorial membrane morphology / MGI
detached tectorial membrane / MGI
abnormal cochlear microphonics / MGI
decreased cochlear microphonics / MGI
decreased cochlear nerve compound action potential / MGI
abnormal hair cell mechanoelectric transduction / MGI
abnormal cochlear outer hair cell physiology / MGI
abnormal cochlear hair cell stereociliary bundle morphology / MGI
abnormal distortion product otoacoustic emission / MGI
absent distortion product otoacoustic emissions / MGI
hearing/vestibular/ear phenotype / MGI
behavior/neurological phenotype / MGI
impaired hearing / MGI
abnormal otoacoustic response / MGI
increased or absent threshold for auditory brainstem response / MGI
abnormal Hensen stripe morphology / MGI
absent Hensen stripe / MGI
abnormal Kimura membrane morphology / MGI
abnormal tectorial membrane marginal band morphology / MGI
abnormal tectorial membrane covernet morphology / MGI
abnormal tectorial membrane striated-sheet matrix morphology / MGI

Literature references

A targeted deletion in alpha-tectorin reveals that the tectorial membrane is required for the gain and timing of cochlear feedback.;Legan P K, Lukashkina V A, Goodyear R J, Kössi M, Russell I J, Richardson G P, ;2000;Neuron;28;273-85; 11087000

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6.129S-Tectatm1Gpr/H