C57BL/6NTac-Pik3cbtm1a(EUCOMM)Wtsi/WtsiOulu

Status

Available to order

EMMA IDEM:05526
International strain nameC57BL/6NTac-Pik3cbtm1a(EUCOMM)Wtsi/WtsiOulu
Alternative nameEPD0051_3_E05
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPik3cbtm1a(EUCOMM)Wtsi
Gene/Transgene symbolPik3cb
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0051_3_E05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal coat/hair pigmentation / IMPC
  • decreased circulating creatinine level / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • increased hemoglobin content / IMPC
  • hypoplasia / IMPC
IMPC phenotypes (gene matching)
  • increased hemoglobin content / IMPC
  • hypoplasia / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • decreased circulating creatinine level / IMPC
  • abnormal coat/hair pigmentation / IMPC
MGI phenotypes (allele matching)
  • decreased circulating creatinine level / MGI
  • increased mean corpuscular hemoglobin / MGI
  • increased hemoglobin content / MGI
  • decreased lumbar vertebrae number / MGI
MGI phenotypes (gene matching)
  • decreased body weight / MGI
  • weight loss / MGI
  • decreased embryo size / MGI
  • postnatal growth retardation / MGI
  • decreased circulating triglyceride level / MGI
  • increased insulin secretion / MGI
  • abnormal gluconeogenesis / MGI
  • decreased lumbar vertebrae number / MGI
  • decreased circulating cholesterol level / MGI
  • insulin resistance / MGI
  • decreased heart rate / MGI
  • decreased glycogen level / MGI
  • abnormal platelet physiology / MGI
  • pancreatic islet hyperplasia / MGI
  • decreased circulating creatinine level / MGI
  • increased circulating glucose level / MGI
  • increased mean corpuscular hemoglobin / MGI
  • increased hemoglobin content / MGI
  • increased lung weight / MGI
  • pulmonary fibrosis / MGI
  • decreased physiological sensitivity to xenobiotic / MGI
  • decreased platelet aggregation / MGI
  • decreased birth body size / MGI
  • decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
  • preweaning lethality, complete penetrance / MGI
  • embryonic lethality, incomplete penetrance / MGI
  • embryonic lethality before implantation, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • decreased fibroblast proliferation / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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