C57BL/6NTac-Optntm1a(EUCOMM)Wtsi/WtsiOulu

Status

Available to order

EMMA IDEM:05525
International strain nameC57BL/6NTac-Optntm1a(EUCOMM)Wtsi/WtsiOulu
Alternative nameEPD0116_2_G06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolOptntm1a(EUCOMM)Wtsi
Gene/Transgene symbolOptn
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0116_2_G06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • Functional analysis of candidate genes from genome-wide association studies of hearing.;Ingham Neil J, Rook Victoria, Di Domenico Francesca, James Elysia, Lewis Morag A, Girotto Giorgia, Buniello Annalisa, Steel Karen P, ;2020;Hearing research;387;107879; 31927188

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased heart weight / IMPC
IMPC phenotypes (gene matching)
  • decreased heart weight / IMPC
MGI phenotypes (allele matching)
  • decreased circulating LDL cholesterol level / MGI
  • decreased circulating HDL cholesterol level / MGI
  • decreased circulating cholesterol level / MGI
  • decreased circulating potassium level / MGI
MGI phenotypes (gene matching)
  • decreased circulating LDL cholesterol level / MGI
  • decreased circulating HDL cholesterol level / MGI
  • no phenotypic analysis / MGI
  • decreased circulating cholesterol level / MGI
  • immune system phenotype / MGI
  • decreased circulating potassium level / MGI
  • mortality/aging / MGI
  • embryonic lethality, incomplete penetrance / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • Functional analysis of candidate genes from genome-wide association studies of hearing.;Ingham Neil J, Rook Victoria, Di Domenico Francesca, James Elysia, Lewis Morag A, Girotto Giorgia, Buniello Annalisa, Steel Karen P, ;2020;Hearing research;387;107879; 31927188
  • Ubiquitylation of autophagy receptor Optineurin by HACE1 activates selective autophagy for tumor suppression.;Liu Zhengzhao, Chen Peng, Gao Hong, Gu Yu, Yang Jiao, Peng Hong, Xu Xingxing, Wang Haifeng, Yang Meiqiang, Liu Xiaoying, Fan Libin, Chen Shiyao, Zhou Jian, Sun Yihong, Ruan Kangchen, Cheng Shuqun, Komatsu Masaaki, White Eileen, Li Lin, Ji Hongbin, Finley Daniel, Hu Ronggui, ;2014;Cancer cell;26;106-20; 25026213

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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