C57BL/6NTac-Plin2^{tm1a(EUCOMM)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:05522
Citation information	RRID:IMSR_EM:05522 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Plin2^{tm1a(EUCOMM)Wtsi}/WtsiOulu
Alternative name	EPD0045_1_D02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Plin2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Plin2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0045_1_D02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating total protein level / IMPC

IMPC phenotypes (gene matching)

increased circulating total protein level / IMPC

MGI phenotypes (gene matching)

decreased body weight / MGI
hyperactivity / MGI
decreased inflammatory response / MGI
male infertility / MGI
decreased susceptibility to hepatic steatosis / MGI
decreased circulating triglyceride level / MGI
decreased circulating free fatty acid level / MGI
abnormal sebaceous gland physiology / MGI
delayed dark adaptation / MGI
abnormal milk composition / MGI
adipose tissue phenotype / MGI
homeostasis/metabolism phenotype / MGI
vision/eye phenotype / MGI
abnormal eye electrophysiology / MGI
decreased susceptibility to diet-induced obesity / MGI
abnormal adipose tissue physiology / MGI
decreased circulating leptin level / MGI
abnormal maternal behavior / MGI
decreased subcutaneous adipose tissue amount / MGI
abnormal white fat cell morphology / MGI
decreased fat cell size / MGI
decreased epididymal fat pad weight / MGI
decreased liver triglyceride level / MGI
decreased total body fat amount / MGI
abnormal vitamin A metabolism / MGI
decreased food intake / MGI
small sebaceous gland / MGI
sebaceous gland hypoplasia / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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