C57BL/6NTac-Myd88^{tm1a(EUCOMM)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:05518
International strain name	C57BL/6NTac-Myd88^{tm1a(EUCOMM)Wtsi}/WtsiOulu
Alternative name	EPD0226_1_D04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Myd88^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Myd88
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0226_1_D04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Waldenström macroglobulinemia / Orphanet_33226
Bacterial susceptibility due to TLR signaling pathway deficiency / Orphanet_183713

IMPC phenotypes (allele matching)

abnormal behavior / IMPC
inflammation / IMPC

IMPC phenotypes (gene matching)

inflammation / IMPC
abnormal behavior / IMPC

MGI phenotypes (allele matching)

increased body weight / MGI
decreased mean corpuscular volume / MGI

MGI phenotypes (gene matching)

decreased neutrophil cell number / MGI
abnormal enterocyte morphology / MGI
increased body weight / MGI
hyperglycemia / MGI
abnormal immune system physiology / MGI
decreased IgG level / MGI
decreased inflammatory response / MGI
abnormal glucose homeostasis / MGI
abnormal respiratory system physiology / MGI
abnormal airway responsiveness / MGI
abnormal dendritic cell physiology / MGI
increased susceptibility to bacterial infection / MGI
increased susceptibility to viral infection / MGI
abnormal innate immunity / MGI
abnormal adaptive immunity / MGI
abnormal cell-mediated immunity / MGI
abnormal T cell physiology / MGI
abnormal macrophage physiology / MGI
abnormal neutrophil physiology / MGI
increased IgA level / MGI
increased IgE level / MGI
decreased mean corpuscular volume / MGI
decreased circulating alanine transaminase level / MGI
abnormal cytokine secretion / MGI
no phenotypic analysis / MGI
rectal hemorrhage / MGI
decreased susceptibility to induced arthritis / MGI
abnormal pancreatic beta cell physiology / MGI
abnormal nervous system physiology / MGI
decreased susceptibility to kidney reperfusion injury / MGI
decreased incidence of tumors by chemical induction / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
abnormal response to infection / MGI
decreased susceptibility to parasitic infection / MGI
decreased B cell proliferation / MGI
abnormal pancreatic beta cell morphology / MGI
digestive/alimentary phenotype / MGI
immune system phenotype / MGI
reproductive system phenotype / MGI
hematopoietic system phenotype / MGI
decreased circulating aspartate transaminase level / MGI
increased apoptosis / MGI
abnormal retinal progenitor cell morphology / MGI
abnormal susceptibility to hearing loss / MGI
abnormal NK T cell physiology / MGI
abnormal dendritic cell differentiation / MGI
increased dendritic cell number / MGI
decreased IgG2b level / MGI
decreased IgG3 level / MGI
increased IgG1 level / MGI
increased susceptibility to induced colitis / MGI
decreased circulating tumor necrosis factor level / MGI
decreased tumor necrosis factor secretion / MGI
decreased interferon-alpha secretion / MGI
decreased interferon-gamma secretion / MGI
decreased circulating interleukin-10 level / MGI
decreased circulating interleukin-6 level / MGI
decreased circulating interleukin-12b level / MGI
decreased interleukin-1 beta secretion / MGI
decreased interleukin-10 secretion / MGI
decreased interleukin-12 secretion / MGI
decreased interleukin-12b secretion / MGI
decreased interleukin-6 secretion / MGI
abnormal cytokine level / MGI
abnormal chemokine level / MGI
abnormal chemokine secretion / MGI
abnormal interferon level / MGI
abnormal tumor necrosis factor level / MGI
increased physiological sensitivity to xenobiotic / MGI
abnormal enterocyte physiology / MGI
decreased pancreatic beta cell mass / MGI
small pancreatic islets / MGI
abnormal neuron differentiation / MGI
decreased liver tumor incidence / MGI
abnormal gut flora balance / MGI
abnormal NK cell physiology / MGI
altered susceptibility to bacterial infection / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Myd88tm1a(EUCOMM)Wtsi/WtsiOulu