C57BL/6N-Tlr9tm1.2Arte/Ieg

Status

Available to order

EMMA IDEM:05496
International strain nameC57BL/6N-Tlr9tm1.2Arte/Ieg
Alternative nameTlr9 KO conv
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolTlr9tm1.2
Gene/Transgene symbolTlr9

Information from provider

Provider Helmholtz Zentrum Muenchen
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen German Research Center for Environmental Health (GmbH)
Genetic informationExon 2 has been flanked by loxP sites, as its genetic ablation should result in loss of function by deletion of most of the open reading frame (see EMMA strain EM:05497). The constitutive knock-out mutation has been obtained by in vivo cre recombinase-mediated deletion.
Phenotypic informationTO BE PROVIDED
Breeding historyTargeting performed in C57BL/6N ES cells and confirmed by Southern blotting. Tlr9 floxed mice were crossed to C57BL/6N cre-deleter mice to obtain the Tlr9 null allele. Maintained on C57BL/6N background.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac, wild-type C57BL/6N
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal immune system physiology / MGI
  • decreased inflammatory response / MGI
  • abnormal dendritic cell physiology / MGI
  • increased susceptibility to viral infection / MGI
  • abnormal macrophage physiology / MGI
  • abnormal B cell physiology / MGI
  • abnormal cytokine secretion / MGI
  • abnormal leukocyte migration / MGI
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • abnormal response to infection / MGI
  • decreased susceptibility to parasitic infection / MGI
  • impaired natural killer cell mediated cytotoxicity / MGI
  • decreased B cell proliferation / MGI
  • immune system phenotype / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal NK T cell physiology / MGI
  • increased susceptibility to induced colitis / MGI
  • decreased tumor necrosis factor secretion / MGI
  • decreased interferon-alpha secretion / MGI
  • decreased interferon-beta secretion / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased interleukin-12b secretion / MGI
  • abnormal plasmacytoid dendritic cell physiology / MGI
  • abnormal NK cell physiology / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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