MGI phenotypes (allele matching)
B6N.Cg-Piwil4tm1.1Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:05453 |
| Citation information | RRID:IMSR_EM:05453 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N.Cg-Piwil4tm1.1Doca/Cnrm |
| Alternative name | Miwi2 DAH |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Piwil4tm1.1Doca |
| Gene/Transgene symbol | Piwil4 |
Information from provider
| Provider | Donal O'Carroll |
| Provider affiliation | EMBL Mouse Biology Unit |
| Genetic information | To generate Miwi2 D761A allele the wild-type exon 17 was replaced with a mutant exon where the aspartic acid 761 codon is mutated to encode an alanine. A targeting construct was recombineered that contains homology arms, an frt flanked neomycin (neo) cassette 3' of exon 17 that contains the Miwi2 D761A mutation; flp-mediated recombination caused an excision of the Neor-frt flanked cassette that results in the Miwi2 DAH allele. |
| Phenotypic information | Homozygous male and female mice for Miwi2 DAH allele are viable, healthy and fertile. Miwi2 DAH mice do not present any apparent abnormalities. |
| Breeding history | Mice carrying the targeted allele were crossed with flp-expressing transgenic mice (FlpeR) to remove the Frt-flanked Neor cassette. The resulting offspring was backcrossed to C57BL/6N to remove the FLP transgene. Mice carrying the mutation were then backcrossed 8 generations to C57BL/6N. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (gene matching)
Literature references
- The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements.;De Fazio Serena, Bartonicek Nenad, Di Giacomo Monica, Abreu-Goodger Cei, Sankar Aditya, Funaya Charlotta, Antony Claude, Moreira Pedro N, Enright Anton J, O'Carroll Dónal, ;2011;Nature;480;259-63; 22020280