B6.129P2-Tacr1^tm1Sph/H

Status	Available to order
EMMA ID	EM:05452
Citation information	RRID:IMSR_EM:05452 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Tacr1^tm1Sph/H
Alternative name	NK1 Receptor knock-out / Backcrossed on C57BL/6
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Tacr1^tm1Sph
Gene/Transgene symbol	Tacr1

Information from provider

Provider	S P Hunt
Provider affiliation	Windeyer Institute of Medical Sciences, University College London
Genetic information	Targeting construct for NK1 (or Tacr1) receptor is derived from 129/Sv DNA. A reporter/selection cassette (IRES-Gal-MC1-Neo) was inserted into exon 1 of the targeted allele, rendering the mRNA out-of-frame and thus no functional NK1 receptor can be produced.
Phenotypic information	Deletion of Tacr1/NK1 receptor gene. Viable, normal breeding pattern. No obvious phenotype.
Breeding history	Backcrossed for more than 10 generations.
References	Altered nociception, analgesia and aggression in mice lacking the receptor for substance P.;De Felipe C, Herrero J F, O'Brien J A, Palmer J A, Doyle C A, Smith A J, Laird J M, Belmonte C, Cervero F, Hunt S P, ;1998;Nature;392;394-7; 9537323 Genetic background influences the behavioural and molecular consequences of neurokinin-1 receptor knockout.;McCutcheon J E, Fisher A S, Guzdar E, Wood S A, Lightman S L, Hunt S P, ;2008;The European journal of neuroscience;27;683-90; 18279320
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6 males
Breeding at archiving centre	Males were archived upon arrival at the archiving centre. No breeding was carried out.

Disease and phenotype information

MGI allele-associated human disease models

attention deficit hyperactivity disorder / DOID:1094

MGI phenotypes (allele matching)

hyperactivity / MGI
impaired coordination / MGI
abnormal dopamine level / MGI
abnormal pain threshold / MGI
abnormal noradrenaline level / MGI
impaired conditioned place preference behavior / MGI
impaired behavioral response to addictive substance / MGI
impaired behavioral response to morphine / MGI
abnormal nervous system physiology / MGI
abnormal thermosensation / MGI
decreased inflammatory response / MGI
increased airway responsiveness / MGI
abnormal touch/ nociception / MGI
abnormal respiratory mucosa goblet cell morphology / MGI
abnormal chemical nociception / MGI
joint inflammation / MGI
decreased nerve fiber response intensity / MGI
hyperalgesia / MGI
abnormal pulmonary respiratory rate / MGI
decreased aggression / MGI

MGI phenotypes (gene matching)

decreased anxiety-related response / MGI
hyperactivity / MGI
impaired coordination / MGI
abnormal body temperature homeostasis / MGI
abnormal humoral immune response / MGI
decreased inflammatory response / MGI
abnormal dopamine level / MGI
increased airway responsiveness / MGI
abnormal touch/ nociception / MGI
abnormal pain threshold / MGI
abnormal nervous system electrophysiology / MGI
abnormal respiratory mucosa goblet cell morphology / MGI
abnormal mast cell physiology / MGI
decreased circulating corticosterone level / MGI
abnormal chemical nociception / MGI
abnormal nociception after inflammation / MGI
joint inflammation / MGI
decreased vascular permeability / MGI
decreased nerve fiber response intensity / MGI
abnormal nervous system physiology / MGI
abnormal thermosensation / MGI
abnormal noradrenaline level / MGI
abnormal neuron physiology / MGI
hyperalgesia / MGI
hyporesponsive to tactile stimuli / MGI
decreased aggression / MGI
increased chemical nociceptive threshold / MGI
abnormal physiological response to xenobiotic / MGI
impaired conditioned place preference behavior / MGI
impaired behavioral response to xenobiotic / MGI
impaired behavioral response to addictive substance / MGI
impaired behavioral response to morphine / MGI
decreased vocalization / MGI
abnormal pulmonary respiratory rate response / MGI

Literature references

Altered nociception, analgesia and aggression in mice lacking the receptor for substance P.;De Felipe C, Herrero J F, O'Brien J A, Palmer J A, Doyle C A, Smith A J, Laird J M, Belmonte C, Cervero F, Hunt S P, ;1998;Nature;392;394-7; 9537323
Genetic background influences the behavioural and molecular consequences of neurokinin-1 receptor knockout.;McCutcheon J E, Fisher A S, Guzdar E, Wood S A, Lightman S L, Hunt S P, ;2008;The European journal of neuroscience;27;683-90; 18279320

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6.129P2-Tacr1tm1Sph/H