B6Dnk;B6Brd;B6N-Tyr^c-Brd Cyb561^{tm1a(EUCOMM)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:05437
International strain name	B6Dnk;B6Brd;B6N-Tyr^c-Brd Cyb561^{tm1a(EUCOMM)Wtsi}/WtsiOulu
Alternative name	EPD0037_3_G01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cyb561^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cyb561
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0037_3_G01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased circulating calcium level / IMPC
abnormal response to new environment / IMPC
abnormal coat/hair pigmentation / IMPC
increased hematocrit / IMPC
abnormal bone mineralization / IMPC
decreased circulating chloride level / IMPC
increased erythrocyte cell number / IMPC
abnormal bone structure / IMPC
abnormal behavior / IMPC
decreased circulating serum albumin level / IMPC
increased hemoglobin content / IMPC
decreased circulating total protein level / IMPC
abnormal placement of pupils / IMPC
increased circulating amylase level / IMPC
increased circulating iron level / IMPC
increased circulating magnesium level / IMPC

IMPC phenotypes (gene matching)

abnormal response to new environment / IMPC
increased erythrocyte cell number / IMPC
decreased circulating total protein level / IMPC
decreased fasting circulating glucose level / IMPC
decreased lean body mass / IMPC
decreased circulating chloride level / IMPC
increased heart weight / IMPC
increased circulating aspartate transaminase level / IMPC
decreased bone mineral content / IMPC
abnormal head size / IMPC
increased total body fat amount / IMPC
abnormal bone structure / IMPC
abnormal behavior / IMPC
abnormal cranium morphology / IMPC
increased circulating iron level / IMPC
increased circulating amylase level / IMPC
narrow eye opening / IMPC
decreased circulating glucose level / IMPC
abnormal iris morphology / IMPC
tremors / IMPC
decreased exploration in new environment / IMPC
decreased circulating serum albumin level / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased circulating calcium level / IMPC
abnormal tail length / IMPC
abnormal retina blood vessel morphology / IMPC
increased hematocrit / IMPC
abnormal gait / IMPC
increased circulating magnesium level / IMPC
abnormal placement of pupils / IMPC
abnormal coat/hair pigmentation / IMPC
abnormal maxilla morphology / IMPC
enlarged heart / IMPC
increased hemoglobin content / IMPC
increased circulating triglyceride level / IMPC
abnormal bone mineralization / IMPC
abnormal snout morphology / IMPC

MGI phenotypes (allele matching)

decreased circulating LDL cholesterol level / MGI
abnormal coat/hair pigmentation / MGI
increased hematocrit / MGI
increased erythrocyte cell number / MGI
abnormal behavior / MGI
increased hemoglobin content / MGI
increased circulating amylase level / MGI
increased circulating fructosamine level / MGI
increased circulating magnesium level / MGI

MGI phenotypes (gene matching)

decreased circulating LDL cholesterol level / MGI
abnormal coat/hair pigmentation / MGI
increased hematocrit / MGI
increased erythrocyte cell number / MGI
abnormal behavior / MGI
increased hemoglobin content / MGI
increased circulating amylase level / MGI
increased circulating fructosamine level / MGI
increased circulating magnesium level / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6Brd;B6N-Tyrc-Brd Cyb561tm1a(EUCOMM)Wtsi/WtsiOulu