B6Brd;B6N-Tyr^c-Brd A^tm1Brd Cadm1^{tm1a(EUCOMM)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:05436
International strain name	B6Brd;B6N-Tyr^c-Brd A^tm1Brd Cadm1^{tm1a(EUCOMM)Wtsi}/WtsiOulu
Alternative name	EPD0126_4_E06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cadm1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cadm1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0126_4_E06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal retina morphology / IMPC
abnormal tail movements / IMPC
abnormal skin pigmentation / IMPC
abnormal behavior / IMPC
abnormal retina pigmentation / IMPC
process of degenerative change / IMPC

IMPC phenotypes (gene matching)

process of degenerative change / IMPC
abnormal behavior / IMPC
abnormal retina morphology / IMPC
abnormal retina pigmentation / IMPC
abnormal skin pigmentation / IMPC
abnormal tail movements / IMPC
decreased grip strength / IMPC

MGI phenotypes (allele matching)

abnormal tail movements / MGI
abnormal behavior / MGI

MGI phenotypes (gene matching)

abnormal hippocampus morphology / MGI
arrest of spermatogenesis / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal tail movements / MGI
male infertility / MGI
abnormal T cell differentiation / MGI
abnormal brain morphology / MGI
no abnormal phenotype detected / MGI
abnormal astrocyte morphology / MGI
asthenozoospermia / MGI
globozoospermia / MGI
oligozoospermia / MGI
nervous system phenotype / MGI
increased pancreatic beta cell number / MGI
decreased fetal size / MGI
abnormal testis weight / MGI
decreased testis weight / MGI
decreased male germ cell number / MGI
abnormal behavior / MGI
increased double-positive T cell number / MGI
decreased T cell proliferation / MGI
endocrine/exocrine gland phenotype / MGI
teratozoospermia / MGI
abnormal spermatid morphology / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
arrest of spermiogenesis / MGI
abnormal neocortex morphology / MGI
decreased interferon-gamma secretion / MGI
decreased interleukin-2 secretion / MGI
decreased interleukin-4 secretion / MGI
abnormal T cell receptor V(D)J recombination / MGI
increased pancreatic beta cell mass / MGI
short sperm flagellum / MGI
multiflagellated sperm / MGI
increased pancreatic beta cell proliferation / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Atm1Brd Cadm1tm1a(EUCOMM)Wtsi/WtsiOulu