B6Dnk;B6N-Epc2tm1e(EUCOMM)Wtsi/WtsiOulu

Status

Available to order

EMMA IDEM:05434
International strain nameB6Dnk;B6N-Epc2tm1e(EUCOMM)Wtsi/WtsiOulu
Alternative nameEPD0013_1_B09
Strain typeTargeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbolEpc2tm1e(EUCOMM)Wtsi
Gene/Transgene symbolEpc2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0013_1_B09. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)
  • preweaning lethality, complete penetrance / IMPC
IMPC phenotypes (gene matching)
  • abnormal retina morphology / IMPC
  • abnormal lens morphology / IMPC
  • increased respiratory quotient / IMPC
  • decreased total retina thickness / IMPC
  • abnormal behavior / IMPC
  • increased pulmonary respiratory rate / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • cataract / IMPC
  • abnormal retina pigmentation / IMPC
  • decreased locomotor activity / IMPC
  • thrombocytopenia / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased thigmotaxis / IMPC
  • abnormal eye morphology / IMPC
  • decreased lactate dehydrogenase level / IMPC
  • abnormal retina blood vessel morphology / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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