B6Dnk;B6Brd;B6N-Tyr^c-Brd Arid2^{tm1a(EUCOMM)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:05427
International strain name	B6Dnk;B6Brd;B6N-Tyr^c-Brd Arid2^{tm1a(EUCOMM)Wtsi}/WtsiOulu
Alternative name	EPD0027_2_B05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Arid2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Arid2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0027_2_B05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Coffin-Siris syndrome / Orphanet_1465

IMPC phenotypes (allele matching)

preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

abnormal angiogenesis / MGI
double outlet right ventricle / MGI
abnormal cardiovascular system physiology / MGI
hemorrhage / MGI
abnormal atrioventricular valve morphology / MGI
thin myocardium compact layer / MGI
abnormal coronary artery morphology / MGI
cardiovascular system phenotype / MGI
immune system phenotype / MGI
inlet ventricular septal defect / MGI
common atrioventricular valve / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
decreased fetal cardiomyocyte proliferation / MGI
subcutaneous edema / MGI

MGI phenotypes (gene matching)

abnormal angiogenesis / MGI
double outlet right ventricle / MGI
abnormal cardiovascular system physiology / MGI
hemorrhage / MGI
abnormal atrioventricular valve morphology / MGI
thin myocardium compact layer / MGI
abnormal coronary artery morphology / MGI
cardiovascular system phenotype / MGI
immune system phenotype / MGI
inlet ventricular septal defect / MGI
common atrioventricular valve / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
decreased fetal cardiomyocyte proliferation / MGI
subcutaneous edema / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
BAF200 is required for heart morphogenesis and coronary artery development.;He Lingjuan, Tian Xueying, Zhang Hui, Hu Tianyuan, Huang Xiuzhen, Zhang Libo, Wang Zhong, Zhou Bin, ;2014;PloS one;9;e109493; 25299188
Arid2 regulates hematopoietic stem cell differentiation in normal hematopoiesis.;Bluemn Theresa, Schmitz Jesse, Chen Yuhong, Zheng Yongwei, Zhang Yongguang, Zheng Shikan, Burns Robert, DeJong Joshua, Christiansen Luke, Izaguirre-Carbonell Jesus, Wang Demin, Zhu Nan, ;2021;Experimental hematology;94;37-46; 33346030
Differential roles of BAF and PBAF subunits, Arid1b and Arid2, in MLL-AF9 leukemogenesis.;Bluemn Theresa, Schmitz Jesse, Zheng Yongwei, Burns Robert, Zheng Shikan, DeJong Joshua, Christiansen Luke, Arnold Olivia, Izaguirre-Carbonell Jesus, Wang Demin, Deshpande Aniruddha J, Zhu Nan, ;2022;Leukemia;36;946-955; 35022500

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6Brd;B6N-Tyrc-Brd Arid2tm1a(EUCOMM)Wtsi/WtsiOulu