C57BL/6N-Hprt1^{tm1(CMV-cre)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:05414
International strain name	C57BL/6N-Hprt1^{tm1(CMV-cre)Wtsi}/WtsiOulu
Alternative name	HPRT CMV Cre (CRET)
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Hprt1^{tm1(CMV-cre)Wtsi}
Gene/Transgene symbol	Hprt1

Information from provider

Provider	Haydn Prosser
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	Generation of germline cre recombinase deleter in C57BL/6N JM8.F6 embryonic stem cells. Allele symbol: Hprt1^{tm1(CMV-cre)Wtsi}; short name CMV-Cre.
Phenotypic information	Germline cre recombinase deleter.
Breeding history	Inbred in C57BL/6NTac
References	A targeted X-linked CMV-Cre line.;Su Hong, Mills Alea A, Wang Xiaozhong, Bradley Allan, ;2002;Genesis (New York, N.Y. : 2000);32;187-8; 11857817 A new mutation of Sgms1 causes gradual hearing loss associated with a reduced endocochlear potential.;Chen Jing, Lewis Morag A, Wai Alisa, Yin Lucia, Dawson Sally J, Ingham Neil J, Steel Karen P, ;2024;Hearing research;451;109091; 39067415 The role of sphingosine-1-phosphate transporter Spns2 in immune system function.;Nijnik Anastasia, Clare Simon, Hale Christine, Chen Jing, Raisen Claire, Mottram Lynda, Lucas Mark, Estabel Jeanne, Ryder Edward, Adissu Hibret, null null, Adams Niels C, Ramirez-Solis Ramiro, White Jacqueline K, Steel Karen P, Dougan Gordon, Hancock Robert E W, ;2012;Journal of immunology (Baltimore, Md. : 1950);189;102-11; 22664872 Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene.;Maguire Simon, Estabel Jeanne, Ingham Neil, Pearson Selina, Ryder Edward, Carragher Damian M, Walker Nicolas, null null, Bussell James, Chan Wai-In, Keane Thomas M, Adams David J, Scudamore Cheryl L, Lelliott Christopher J, Ramírez-Solis Ramiro, Karp Natasha A, Steel Karen P, White Jacqueline K, Gerdin Anna-Karin, ;2014;PloS one;9;e91807; 24642684 Spinster homolog 2 (spns2) deficiency causes early onset progressive hearing loss.;Chen Jing, Ingham Neil, Kelly John, Jadeja Shalini, Goulding David, Pass Johanna, Mahajan Vinit B, Tsang Stephen H, Nijnik Anastasia, Jackson Ian J, White Jacqueline K, Forge Andrew, Jagger Daniel, Steel Karen P, ;2014;PLoS genetics;10;e1004688; 25356849 Alternative Splice Forms Influence Functions of Whirlin in Mechanosensory Hair Cell Stereocilia.;Ebrahim Seham, Ingham Neil J, Lewis Morag A, Rogers Michael J C, Cui Runjia, Kachar Bechara, Pass Johanna C, Steel Karen P, ;2016;Cell reports;15;935-943; 27117407
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (gene matching)

increased mean corpuscular volume / IMPC
thrombocytopenia / IMPC
increased circulating HDL cholesterol level / IMPC
decreased erythrocyte cell number / IMPC
abnormal gait / IMPC

MGI phenotypes (gene matching)

decreased hematocrit / MGI
increased leukocyte cell number / MGI
increased neutrophil cell number / MGI
abnormal small intestine morphology / MGI
abnormal liver morphology / MGI
abnormal branching of the mammary ductal tree / MGI
enlarged spleen / MGI
spleen hyperplasia / MGI
enlarged lymph nodes / MGI
tremors / MGI
convulsive seizures / MGI
abnormal lung morphology / MGI
decreased body weight / MGI
decreased anxiety-related response / MGI
ataxia / MGI
hypoactivity / MGI
impaired coordination / MGI
abnormal gait / MGI
short stride length / MGI
decreased exploration in new environment / MGI
limb grasping / MGI
abnormal motor coordination/balance / MGI
abnormal hematopoietic system physiology / MGI
hyperglycemia / MGI
anemia / MGI
cardiac hypertrophy / MGI
increased mammary adenocarcinoma incidence / MGI
abnormal reflex / MGI
seizures / MGI
abnormal motor capabilities/coordination/movement / MGI
premature death / MGI
abnormal definitive hematopoiesis / MGI
abnormal brain morphology / MGI
no abnormal phenotype detected / MGI
neurodegeneration / MGI
abnormal spleen white pulp morphology / MGI
abnormal hematopoietic system morphology/development / MGI
abnormal megakaryocyte progenitor cell morphology / MGI
hepatic steatosis / MGI
decreased vertical activity / MGI
increased heart weight / MGI
increased systemic arterial blood pressure / MGI
albuminuria / MGI
decreased erythrocyte cell number / MGI
increased urine protein level / MGI
impaired social transmission of food preference / MGI
no phenotypic analysis / MGI
phenotypic reversion / MGI
abnormal dopaminergic neuron morphology / MGI
astrocytosis / MGI
abnormal depression-related behavior / MGI
decreased tumor growth/size / MGI
abnormal nervous system morphology / MGI
abnormal cardiac muscle relaxation / MGI
neuronal intranuclear inclusions / MGI
abnormal myocardial fiber physiology / MGI
abnormal Paneth cell morphology / MGI
decreased B cell number / MGI
decreased cardiac muscle contractility / MGI
glomerulosclerosis / MGI
abnormal podocyte morphology / MGI
muscle phenotype / MGI
homeostasis/metabolism phenotype / MGI
endocrine/exocrine gland phenotype / MGI
behavior/neurological phenotype / MGI
immune system phenotype / MGI
taste/olfaction phenotype / MGI
hematopoietic system phenotype / MGI
jerky movement / MGI
thrombocytosis / MGI
decreased ventricle muscle contractility / MGI
decreased mean corpuscular hemoglobin concentration / MGI
decreased dopamine level / MGI
abnormal podocyte slit diaphragm morphology / MGI
absent podocyte slit diaphragm / MGI
podocyte foot process effacement / MGI
increased megakaryocyte cell number / MGI
abnormal spatial reference memory / MGI
abnormal spatial working memory / MGI
abnormal splenic cell ratio / MGI
abnormal physiological response to xenobiotic / MGI
abnormal enterocyte proliferation / MGI
abnormal enterocyte apoptosis / MGI
abnormal neuron differentiation / MGI
increased mammary gland tumor incidence / MGI
myeloid hyperplasia / MGI
expanded mesangial matrix / MGI
mesangial cell hyperplasia / MGI
abnormal habituation to a new environment / MGI
abnormal ceramide level / MGI
decreased brain choline acetyltransferase activity / MGI
decreased brain tyrosine 3-monooxygenase activity / MGI
decreased vascular endothelial cell proliferation / MGI

Literature references

A targeted X-linked CMV-Cre line.;Su Hong, Mills Alea A, Wang Xiaozhong, Bradley Allan, ;2002;Genesis (New York, N.Y. : 2000);32;187-8; 11857817
A new mutation of Sgms1 causes gradual hearing loss associated with a reduced endocochlear potential.;Chen Jing, Lewis Morag A, Wai Alisa, Yin Lucia, Dawson Sally J, Ingham Neil J, Steel Karen P, ;2024;Hearing research;451;109091; 39067415
The role of sphingosine-1-phosphate transporter Spns2 in immune system function.;Nijnik Anastasia, Clare Simon, Hale Christine, Chen Jing, Raisen Claire, Mottram Lynda, Lucas Mark, Estabel Jeanne, Ryder Edward, Adissu Hibret, null null, Adams Niels C, Ramirez-Solis Ramiro, White Jacqueline K, Steel Karen P, Dougan Gordon, Hancock Robert E W, ;2012;Journal of immunology (Baltimore, Md. : 1950);189;102-11; 22664872
Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene.;Maguire Simon, Estabel Jeanne, Ingham Neil, Pearson Selina, Ryder Edward, Carragher Damian M, Walker Nicolas, null null, Bussell James, Chan Wai-In, Keane Thomas M, Adams David J, Scudamore Cheryl L, Lelliott Christopher J, Ramírez-Solis Ramiro, Karp Natasha A, Steel Karen P, White Jacqueline K, Gerdin Anna-Karin, ;2014;PloS one;9;e91807; 24642684
Spinster homolog 2 (spns2) deficiency causes early onset progressive hearing loss.;Chen Jing, Ingham Neil, Kelly John, Jadeja Shalini, Goulding David, Pass Johanna, Mahajan Vinit B, Tsang Stephen H, Nijnik Anastasia, Jackson Ian J, White Jacqueline K, Forge Andrew, Jagger Daniel, Steel Karen P, ;2014;PLoS genetics;10;e1004688; 25356849
Alternative Splice Forms Influence Functions of Whirlin in Mechanosensory Hair Cell Stereocilia.;Ebrahim Seham, Ingham Neil J, Lewis Morag A, Rogers Michael J C, Cui Runjia, Kachar Bechara, Pass Johanna C, Steel Karen P, ;2016;Cell reports;15;935-943; 27117407

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Hprt1tm1(CMV-cre)Wtsi/WtsiOulu