B6Brd;B6N-Tyr^c-Brd Src^{tm2a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:05380
International strain name	B6Brd;B6N-Tyr^c-Brd Src^{tm2a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0058_2_D10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Src^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Src
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0058_2_D10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6N, wild-type C57BL/6J
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Hereditary thrombocytopenia with early-onset myelofibrosis / Orphanet_480851

IMPC phenotypes (allele matching)

decreased mean corpuscular hemoglobin / IMPC

IMPC phenotypes (gene matching)

decreased mean corpuscular hemoglobin / IMPC

MGI phenotypes (allele matching)

abnormal femur morphology / MGI
abnormal behavior / MGI

MGI phenotypes (gene matching)

abnormal bone marrow cavity morphology / MGI
osteopetrosis / MGI
abnormal tooth eruption / MGI
failure of tooth eruption / MGI
absent incisors / MGI
decreased compact bone thickness / MGI
diluted coat color / MGI
shortened head / MGI
abnormal femur morphology / MGI
decreased body weight / MGI
decreased body size / MGI
delayed eyelid opening / MGI
abnormal social investigation / MGI
hyperactivity / MGI
impaired coordination / MGI
increased startle reflex / MGI
abnormal vocalization / MGI
increased inflammatory response / MGI
female infertility / MGI
abnormal learning/memory/conditioning / MGI
abnormal motor capabilities/coordination/movement / MGI
premature death / MGI
abnormal tooth morphology / MGI
abnormal limb morphology / MGI
abnormal craniofacial bone morphology / MGI
abnormal bone marrow morphology / MGI
increased thigmotaxis / MGI
abnormal motor learning / MGI
delayed endochondral bone ossification / MGI
abnormal response to novel object / MGI
nervous system phenotype / MGI
abnormal long bone morphology / MGI
abnormal bone structure / MGI
abnormal decidualization / MGI
abnormal long bone diaphysis morphology / MGI
decreased length of long bones / MGI
abnormal behavior / MGI
decreased bone resorption / MGI
abnormal anterior eye segment morphology / MGI
hematopoietic system phenotype / MGI
increased long bone epiphyseal plate size / MGI
decreased molar number / MGI
abnormal spatial reference memory / MGI
impaired cued conditioning behavior / MGI
decreased grip strength / MGI
mortality/aging / MGI
abnormal bone trabecula morphology / MGI
increased trabecular bone mass / MGI
postnatal lethality, incomplete penetrance / MGI
abnormal head shape / MGI
abnormal response to social novelty / MGI
abnormal social recognition / MGI
broad face / MGI
periodontium inflammation / MGI
short basicranium / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Srctm2a(EUCOMM)Wtsi/WtsiCnbc