C57BL/6NTac-Atg16l1^{tm1a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:05376
International strain name	C57BL/6NTac-Atg16l1^{tm1a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0102_2_A02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Atg16l1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Atg16l1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0102_2_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6NTac, wild-type C57BL/6J
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

increased circulating serum albumin level / MGI
increased mean corpuscular hemoglobin / MGI
overriding aortic valve / MGI
abnormal eye muscle morphology / MGI
thoracoschisis / MGI
perimembraneous ventricular septal defect / MGI
abnormal hepatic vein morphology / MGI
abnormal inferior vena cava valve morphology / MGI
abnormal intrathoracic topology of vagus nerve / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
absent ductus venosus valve / MGI
abnormal thymus topology / MGI
absent connection between subcutaneous lymph vessels and lymph sac / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI
prenatal lethality / MGI

MGI phenotypes (gene matching)

overriding aortic valve / MGI
prenatal lethality / MGI
increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
increased circulating serum albumin level / MGI
abnormal eye muscle morphology / MGI
abnormal Paneth cell morphology / MGI
digestive/alimentary phenotype / MGI
immune system phenotype / MGI
increased mean corpuscular hemoglobin / MGI
abnormal autophagy / MGI
increased susceptibility to induced colitis / MGI
increased circulating interleukin-1 beta level / MGI
increased interleukin-1 beta secretion / MGI
increased interleukin-18 secretion / MGI
thoracoschisis / MGI
perimembraneous ventricular septal defect / MGI
abnormal hepatic vein morphology / MGI
abnormal inferior vena cava valve morphology / MGI
increased macrophage cytokine production / MGI
neonatal lethality, complete penetrance / MGI
abnormal intrathoracic topology of vagus nerve / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
absent ductus venosus valve / MGI
abnormal thymus topology / MGI
absent connection between subcutaneous lymph vessels and lymph sac / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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