B6Brd;B6N-Tyr^c-Brd Lrig1^{tm1a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:05375
International strain name	B6Brd;B6N-Tyr^c-Brd Lrig1^{tm1a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0070_3_G09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Lrig1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Lrig1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0070_3_G09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131 Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.;DiTommaso Tia, Jones Lynelle K, Cottle Denny L, null null, Gerdin Anna-Karin, Vancollie Valerie E, Watt Fiona M, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, Sundberg John P, White Jacqueline K, Smyth Ian M, ;2014;PLoS genetics;10;e1004705; 25340873

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous 0, wild-type C57BL/6NTac/Den
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal skin condition / IMPC
decreased body length / IMPC
decreased body weight / IMPC
abnormal tail morphology / IMPC
increased heart weight / IMPC
abnormal behavior / IMPC
decreased total body fat amount / IMPC
hyperplasia / IMPC
acanthosis / IMPC

IMPC phenotypes (gene matching)

decreased body weight / IMPC
hyperplasia / IMPC
increased heart weight / IMPC
abnormal skin condition / IMPC
decreased total body fat amount / IMPC
acanthosis / IMPC
abnormal behavior / IMPC
abnormal tail morphology / IMPC
decreased body length / IMPC

MGI phenotypes (allele matching)

distorted hair follicle pattern / MGI
scaly skin / MGI
thick epidermis / MGI
abnormal epidermis stratum basale morphology / MGI
decreased bone mineral density / MGI
abnormal skin condition / MGI
epidermal hyperplasia / MGI
hyperkeratosis / MGI
decreased body weight / MGI
increased susceptibility to bacterial infection / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating alkaline phosphatase level / MGI
vertebral fusion / MGI
fusion of vertebral arches / MGI
abnormal auditory brainstem response / MGI
decreased circulating aspartate transaminase level / MGI
abnormal hair shedding / MGI
decreased total body fat amount / MGI
abnormal skin adnexa morphology / MGI
integument phenotype / MGI
abnormal tail morphology / MGI
abnormal behavior / MGI

MGI phenotypes (gene matching)

decreased bone mineral density / MGI
distorted hair follicle pattern / MGI
alopecia / MGI
thick tail / MGI
abnormal skin condition / MGI
scaly skin / MGI
psoriasis / MGI
thick epidermis / MGI
epidermal hyperplasia / MGI
abnormal epidermis stratum basale morphology / MGI
hyperkeratosis / MGI
decreased body weight / MGI
abnormal skin morphology / MGI
abnormal tail morphology / MGI
no abnormal phenotype detected / MGI
increased susceptibility to bacterial infection / MGI
abnormal keratinocyte differentiation / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating alkaline phosphatase level / MGI
no phenotypic analysis / MGI
parakeratosis / MGI
nervous system phenotype / MGI
abnormal facial morphology / MGI
vertebral fusion / MGI
fusion of vertebral arches / MGI
abnormal distortion product otoacoustic emission / MGI
abnormal auditory brainstem response / MGI
abnormal behavior / MGI
hearing/vestibular/ear phenotype / MGI
immune system phenotype / MGI
decreased circulating aspartate transaminase level / MGI
abnormal hair shedding / MGI
increased keratinocyte proliferation / MGI
decreased total body fat amount / MGI
abnormal skin adnexa morphology / MGI
integument phenotype / MGI
abnormal auditory brainstem response waveform shape / MGI
increased or absent threshold for auditory brainstem response / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.;DiTommaso Tia, Jones Lynelle K, Cottle Denny L, null null, Gerdin Anna-Karin, Vancollie Valerie E, Watt Fiona M, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, Sundberg John P, White Jacqueline K, Smyth Ian M, ;2014;PLoS genetics;10;e1004705; 25340873
LRIG1 engages ligand VISTA and impairs tumor-specific CD8⁺ T cell responses.;Ta Hieu Minh, Roy Dia, Zhang Keman, Alban Tyler, Juric Ivan, Dong Juan, Parthasarathy Prerana B, Patnaik Sachin, Delaney Elizabeth, Gilmour Cassandra, Zakeri Amin, Shukla Nidhi, Rupani Amit, Phoon Yee Peng, Liu Caini, Avril Stefanie, Gastman Brian, Chan Timothy, Wang Li Lily, ;2024;Science immunology;9;eadi7418; 38758807

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6Brd;B6N-Tyrc-Brd Lrig1tm1a(EUCOMM)Wtsi/WtsiCnbc