C57BL/6NTac-Mad2l2tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05374
International strain nameC57BL/6NTac-Mad2l2tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0046_3_B02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolMad2l2tm1a(EUCOMM)Wtsi
Gene/Transgene symbolMad2l2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0046_3_B02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6N, wild-type C57BL/6J
Stage of embryos4/8-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • abnormal foot pad morphology / IMPC
IMPC phenotypes (gene matching)
  • abnormal foot pad morphology / IMPC
MGI phenotypes (gene matching)
  • decreased enterocyte cell number / MGI
  • decreased cell proliferation / MGI
  • weakness / MGI
  • small gonad / MGI
  • abnormal ovary morphology / MGI
  • small ovary / MGI
  • small testis / MGI
  • Leydig cell hyperplasia / MGI
  • seminiferous tubule degeneration / MGI
  • arrest of spermatogenesis / MGI
  • abnormal spermatogenesis / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • decreased embryo size / MGI
  • postnatal growth retardation / MGI
  • male infertility / MGI
  • female infertility / MGI
  • abnormal oogenesis / MGI
  • abnormal seminiferous tubule morphology / MGI
  • absent ovarian follicles / MGI
  • abnormal Sertoli cell morphology / MGI
  • abnormal primordial germ cell migration / MGI
  • abnormal cell cycle / MGI
  • abnormal neuron apoptosis / MGI
  • absent ovary / MGI
  • nervous system phenotype / MGI
  • decreased fetal size / MGI
  • absent germ cells / MGI
  • ovary atrophy / MGI
  • decreased testis weight / MGI
  • decreased male germ cell number / MGI
  • azoospermia / MGI
  • cellular phenotype / MGI
  • reproductive system phenotype / MGI
  • decreased oocyte number / MGI
  • ectopic Sertoli cells / MGI
  • abnormal primordial germ cell morphology / MGI
  • decreased primordial germ cell number / MGI
  • absent primordial germ cells / MGI
  • slow postnatal weight gain / MGI
  • increased enterocyte apoptosis / MGI
  • decreased birth body size / MGI
  • prenatal growth retardation / MGI
  • abnormal double-strand DNA break repair / MGI
  • embryonic lethality, incomplete penetrance / MGI
  • lethality during fetal growth through weaning, incomplete penetrance / MGI
  • abnormal primordial germ cell apoptosis / MGI
  • abnormal epigenetic regulation of gene expression / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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