C57BL/6NTac-Mtor^{tm1a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:05373
Citation information	RRID:IMSR_EM:05373 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Mtor^{tm1a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0046_3_B11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Mtor^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Mtor
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0046_3_B11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6N males, wild-type C57BL/6NCrl females
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Isolated focal cortical dysplasia type IIa / Orphanet_269001
Isolated focal cortical dysplasia type IIb / Orphanet_269008
Macrocephaly-intellectual disability-neurodevelopmental disorder-small thorax syndrome / Orphanet_457485

IMPC phenotypes (allele matching)

decreased blood urea nitrogen level / IMPC
increased circulating sodium level / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

decreased blood urea nitrogen level / IMPC
increased circulating sodium level / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

increased circulating LDL cholesterol level / MGI
decreased circulating LDL cholesterol level / MGI
increased mean corpuscular hemoglobin / MGI
decreased blood urea nitrogen level / MGI

MGI phenotypes (gene matching)

increased circulating LDL cholesterol level / MGI
decreased circulating LDL cholesterol level / MGI
decreased neutrophil cell number / MGI
decreased monocyte cell number / MGI
small spleen / MGI
decreased thymocyte number / MGI
abnormal forebrain morphology / MGI
forebrain hypoplasia / MGI
abnormal diencephalon morphology / MGI
abnormal midbrain morphology / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal gastrulation / MGI
decreased embryo size / MGI
incomplete embryo turning / MGI
embryonic growth arrest / MGI
abnormal humoral immune response / MGI
decreased IgG level / MGI
decreased IgM level / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal extraembryonic tissue morphology / MGI
abnormal muscle physiology / MGI
abnormal B cell differentiation / MGI
no abnormal phenotype detected / MGI
abnormal T cell physiology / MGI
abnormal B cell physiology / MGI
small ectoplacental cone / MGI
no phenotypic analysis / MGI
decreased lean body mass / MGI
embryonic growth retardation / MGI
abnormal embryonic neuroepithelium morphology / MGI
decreased circulating insulin-like growth factor I level / MGI
inner cell mass degeneration / MGI
decreased B cell number / MGI
abnormal trophoblast layer morphology / MGI
abnormal trophoblast giant cell morphology / MGI
decreased double-positive T cell number / MGI
decreased B cell proliferation / MGI
growth/size/body region phenotype / MGI
immune system phenotype / MGI
increased mean corpuscular hemoglobin / MGI
decreased blood urea nitrogen level / MGI
abnormal T cell morphology / MGI
increased CD4-positive, alpha beta T cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
increased CD8-positive, alpha-beta T cell number / MGI
decreased plasma cell number / MGI
decreased follicular B cell number / MGI
decreased marginal zone B cell number / MGI
embryonic lethality / MGI
decreased splenocyte proliferation / MGI
decreased splenocyte number / MGI
decreased splenocyte apoptosis / MGI
decreased transitional stage T1 B cell number / MGI
increased total body fat amount / MGI
decreased skeletal muscle weight / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
preweaning lethality, incomplete penetrance / MGI
absent inner cell mass proliferation / MGI
absent embryonic telencephalon / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Mtortm1a(EUCOMM)Wtsi/WtsiCnbc