C57BL/6NTac-Mtortm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05373
International strain nameC57BL/6NTac-Mtortm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0046_3_B11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolMtortm1a(EUCOMM)Wtsi
Gene/Transgene symbolMtor
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0046_3_B11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6N, wild-type C57BL/6NCrl
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased blood urea nitrogen level / IMPC
  • increased circulating sodium level / IMPC
  • preweaning lethality, complete penetrance / IMPC
IMPC phenotypes (gene matching)
  • decreased blood urea nitrogen level / IMPC
  • increased circulating sodium level / IMPC
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (allele matching)
  • increased circulating LDL cholesterol level / MGI
  • decreased circulating LDL cholesterol level / MGI
  • increased mean corpuscular hemoglobin / MGI
  • decreased blood urea nitrogen level / MGI
MGI phenotypes (gene matching)
  • increased circulating LDL cholesterol level / MGI
  • decreased circulating LDL cholesterol level / MGI
  • decreased neutrophil cell number / MGI
  • decreased monocyte cell number / MGI
  • small spleen / MGI
  • decreased thymocyte number / MGI
  • abnormal forebrain morphology / MGI
  • forebrain hypoplasia / MGI
  • abnormal diencephalon morphology / MGI
  • abnormal midbrain morphology / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • abnormal gastrulation / MGI
  • decreased embryo size / MGI
  • incomplete embryo turning / MGI
  • embryonic growth arrest / MGI
  • abnormal humoral immune response / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • abnormal developmental patterning / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal extraembryonic tissue morphology / MGI
  • abnormal muscle physiology / MGI
  • abnormal B cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • abnormal T cell physiology / MGI
  • abnormal B cell physiology / MGI
  • small ectoplacental cone / MGI
  • no phenotypic analysis / MGI
  • decreased lean body mass / MGI
  • embryonic growth retardation / MGI
  • abnormal embryonic neuroepithelium morphology / MGI
  • decreased circulating insulin-like growth factor I level / MGI
  • inner cell mass degeneration / MGI
  • decreased B cell number / MGI
  • abnormal trophoblast layer morphology / MGI
  • abnormal trophoblast giant cell morphology / MGI
  • decreased double-positive T cell number / MGI
  • decreased B cell proliferation / MGI
  • growth/size/body region phenotype / MGI
  • immune system phenotype / MGI
  • increased mean corpuscular hemoglobin / MGI
  • decreased blood urea nitrogen level / MGI
  • abnormal T cell morphology / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased plasma cell number / MGI
  • decreased follicular B cell number / MGI
  • decreased marginal zone B cell number / MGI
  • embryonic lethality / MGI
  • decreased splenocyte proliferation / MGI
  • decreased splenocyte number / MGI
  • decreased splenocyte apoptosis / MGI
  • decreased transitional stage T1 B cell number / MGI
  • increased total body fat amount / MGI
  • decreased skeletal muscle weight / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality between implantation and somite formation, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • preweaning lethality, incomplete penetrance / MGI
  • absent inner cell mass proliferation / MGI
  • absent embryonic telencephalon / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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