FVB/N-Tg(Rnu6-RNAi:IE-PRV)SH05Gjo/Orl
| Status | Available to order |
| EMMA ID | EM:05365 |
| Citation information | RRID:IMSR_EM:05365 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | FVB/N-Tg(Rnu6-RNAi:IE-PRV)SH05Gjo/Orl |
| Alternative name | SH05 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Rnu6-RNAi:IE-PRV)SH05Gjo |
| Gene/Transgene symbol | Tg(Rnu6-RNAi:IE-PRV)SH05Gjo |
Information from provider
| Provider | Genevieve Jolivet |
| Provider affiliation | INRA, INRA |
| Genetic information | Transgenic mice for a U6-shRNA gene. 4 copies of the transgene are integrated. The U6-shRNA construct encompasses the U6 mouse promoter that drives the transcription of a shRNA expressing transcription unit. The shRNA sequence targets the 3'-part of the intermediate early gene (IE gene) of the Pseudorabies virus (PRV), with the following sequence (5'-CTCCCATCGGGTGAGAAAA-3'). PRV currently infects farm animals (especially pigs) and mice. The U6-shRNA gene is inserted in an empty transgene expression vector encompassing the 5'HS4 insulator from the chicken beta globin gene locus, the human eF1alpha gene promoter (-1430, +2), the rabbit (rb) beta-globin second intron and several enhancers (E1, E2 and E3). The empty expression vector acts as a cargo for the U6-shRNA gene to insure the reliable expression. |
| Phenotypic information | Stable production of siRNA targeting the IE RNA. Resistance to a PRV infection (Bartha strain). No stimulation of the innate immune system in non infected animals. |
| Breeding history | Currently bred with wild-type mice. Stable over 5 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous FVB/N males, wild-type FVB/N females |
Literature references
- Viral infection resistance conferred on mice by siRNA transgenesis.;Daniel-Carlier Nathalie, Sawafta Ashraf, Passet Bruno, Thépot Dominique, Leroux-Coyau Mathieu, Lefèvre François, Houdebine Louis-Marie, Jolivet Geneviève, ;2013;Transgenic research;22;489-500; 22961198