C57BL/6N-Cpe^{tm1a(EUCOMM)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:05350
Citation information	RRID:IMSR_EM:05350 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Cpe^{tm1a(EUCOMM)Hmgu}/Ieg
Alternative name	HEPD0594_7_A02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cpe^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Cpe
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0594_7_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac (USA) males, wild-type C57BL/6NTac (USA) females
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased locomotor activity / IMPC
decreased vertical activity / IMPC
decreased grip strength / IMPC
impaired glucose tolerance / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
aorta stenosis / IMPC
decreased exploration in new environment / IMPC
abnormal locomotor behavior / IMPC
increased circulating alkaline phosphatase level / IMPC
increased startle reflex / IMPC
increased leukocyte cell number / IMPC
increased mean corpuscular volume / IMPC
increased circulating glucose level / IMPC
decreased food intake / IMPC
increased circulating alanine transaminase level / IMPC
decreased body length / IMPC
increased circulating aspartate transaminase level / IMPC
increased fasting circulating glucose level / IMPC
abnormal behavior / IMPC
hyperactivity / IMPC
increased thigmotaxis / IMPC
increased circulating calcium level / IMPC
increased hematocrit / IMPC
decreased circulating chloride level / IMPC
male infertility / IMPC

MGI phenotypes (gene matching)

increased circulating LDL cholesterol level / MGI
hypoglycemia / MGI
decreased digestive secretion / MGI
tremors / MGI
increased body length / MGI
obese / MGI
hypoactivity / MGI
abnormal gait / MGI
polyphagia / MGI
abnormal motor coordination/balance / MGI
abnormal placing response / MGI
increased circulating triglyceride level / MGI
increased circulating HDL cholesterol level / MGI
hyperglycemia / MGI
abnormal hypothalamus secretion / MGI
reduced fertility / MGI
infertility / MGI
abnormal motor capabilities/coordination/movement / MGI
abnormal glucose homeostasis / MGI
increased circulating insulin level / MGI
abnormal muscle physiology / MGI
decreased circulating adrenocorticotropin level / MGI
decreased vertical activity / MGI
abnormal hormone level / MGI
increased pituitary hormone level / MGI
abnormal physical strength / MGI
decreased circulating gastrin level / MGI
increased circulating pituitary hormone level / MGI
increased circulating growth hormone level / MGI
increased growth hormone level / MGI
increased circulating VLDL cholesterol level / MGI
increased circulating cholesterol level / MGI
lethargy / MGI
abnormal pancreatic beta cell morphology / MGI
impaired glucose tolerance / MGI
insulin resistance / MGI
abnormal energy expenditure / MGI
abnormal percent body fat/body weight / MGI
increased circulating triiodothyronine level / MGI
pancreatic islet hyperplasia / MGI
hyporesponsive to tactile stimuli / MGI
increased circulating glucose level / MGI
increased circulating leptin level / MGI
increased pancreatic islet number / MGI
degranulated pancreatic beta cells / MGI
increased total body fat amount / MGI
decreased grip strength / MGI
increased respiratory quotient / MGI
impaired adaptive thermogenesis / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Cpetm1a(EUCOMM)Hmgu/Ieg