C57BL/6N-Cpetm1a(EUCOMM)Hmgu/Ieg

Status

Available to order

EMMA IDEM:05350
International strain nameC57BL/6N-Cpetm1a(EUCOMM)Hmgu/Ieg
Alternative nameHEPD0594_7_A02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolCpetm1a(EUCOMM)Hmgu
Gene/Transgene symbolCpe
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0594_7_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTacUSA, wild-type C57BL/6NTacUSA
Stage of embryos2-cell

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased locomotor activity / IMPC
  • decreased vertical activity / IMPC
  • decreased grip strength / IMPC
  • impaired glucose tolerance / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • aorta stenosis / IMPC
  • decreased exploration in new environment / IMPC
  • abnormal locomotor behavior / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased startle reflex / IMPC
  • increased leukocyte cell number / IMPC
  • increased mean corpuscular volume / IMPC
  • increased circulating glucose level / IMPC
  • decreased food intake / IMPC
  • increased circulating alanine transaminase level / IMPC
  • decreased body length / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • increased fasting circulating glucose level / IMPC
  • abnormal behavior / IMPC
  • hyperactivity / IMPC
  • increased thigmotaxis / IMPC
  • increased circulating calcium level / IMPC
  • increased hematocrit / IMPC
  • decreased circulating chloride level / IMPC
  • male infertility / IMPC
MGI phenotypes (gene matching)
  • increased circulating LDL cholesterol level / MGI
  • hypoglycemia / MGI
  • decreased digestive secretion / MGI
  • tremors / MGI
  • increased body length / MGI
  • obese / MGI
  • hypoactivity / MGI
  • abnormal gait / MGI
  • polyphagia / MGI
  • abnormal motor coordination/balance / MGI
  • abnormal placing response / MGI
  • increased circulating triglyceride level / MGI
  • increased circulating HDL cholesterol level / MGI
  • hyperglycemia / MGI
  • abnormal hypothalamus secretion / MGI
  • reduced fertility / MGI
  • infertility / MGI
  • abnormal motor capabilities/coordination/movement / MGI
  • abnormal glucose homeostasis / MGI
  • increased circulating insulin level / MGI
  • abnormal muscle physiology / MGI
  • decreased circulating adrenocorticotropin level / MGI
  • decreased vertical activity / MGI
  • abnormal hormone level / MGI
  • increased pituitary hormone level / MGI
  • abnormal physical strength / MGI
  • decreased circulating gastrin level / MGI
  • increased circulating pituitary hormone level / MGI
  • increased circulating growth hormone level / MGI
  • increased growth hormone level / MGI
  • increased circulating VLDL cholesterol level / MGI
  • increased circulating cholesterol level / MGI
  • lethargy / MGI
  • abnormal pancreatic beta cell morphology / MGI
  • impaired glucose tolerance / MGI
  • insulin resistance / MGI
  • abnormal energy expenditure / MGI
  • abnormal percent body fat/body weight / MGI
  • increased circulating triiodothyronine level / MGI
  • pancreatic islet hyperplasia / MGI
  • hyporesponsive to tactile stimuli / MGI
  • increased circulating glucose level / MGI
  • increased circulating leptin level / MGI
  • increased pancreatic islet number / MGI
  • degranulated pancreatic beta cells / MGI
  • increased total body fat amount / MGI
  • decreased grip strength / MGI
  • increased respiratory quotient / MGI
  • impaired adaptive thermogenesis / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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