strain-details - INFRAFRONTIER

Information from provider

Status	Available to order
EMMA ID	EM:05339
International strain name	129S9.B6-a/H
Alternative name	129S9/SvEvH.B6-a/H
Strain type	Spontaneous
Allele/Transgene symbol	a
Gene/Transgene symbol	A

Provider	Sara Wells
Provider affiliation	MRC Mammalian Genetics Unit
Genetic information	The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a). When chimaeric mice derived from 129S9/SvEvH ES cells are tested for germ line transmission (GLT) it is often performed against a C57BL/6J background to use the non-agouti phenotype as a marker of non-GLT. Mice with Agouti coats are derived from the ES cells and therefore potential carriers of the genetic alteration. These mice however are F1s between C57BL/6 and 129S9/SvEvH. By transferring the a locus to the 129S9/SvEvH background we be able to maintain the 129 genetic background whilst checking for GLT. This strain allows chimaeric mice derived from 129S9/SvEvH ES cells to be tested for germline transmission using coat colour but ensuring that the genetic background is maintained as 129S9/SvEvH.
Phenotypic information	Black coat colour. The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a).
Breeding history	The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a) and maintained by brother x sister mating.
References	A new coat color mouse line for testing germline transmission of embryonic stem cells while retaining an inbred genetic background.;Barbaric Ivana, Stewart Michelle, Wells Sara, Dear T Neil, ;2007;Journal of the American Association for Laboratory Animal Science : JAALAS;46;37-40; 17487951
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous 0, homozygous 0
Breeding at archiving centre	The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a).
Stage of embryos	2-cell

Disease and phenotype information

MGI allele-associated human disease models

MGI phenotypes (gene matching)

increased brown adipose tissue amount / MGI
irregular coat pigmentation / MGI
mottled coat / MGI
pigmentation phenotype / MGI
increased body length / MGI
increased body weight / MGI
obese / MGI
hypoactivity / MGI
abnormal coat appearance / MGI
hyperglycemia / MGI
increased urine glucose level / MGI
neoplasm / MGI
increased tumor incidence / MGI
decreased incidence of induced tumors / MGI
abnormal coat/hair pigmentation / MGI
increased circulating insulin level / MGI
prenatal lethality / MGI
abnormal skin pigmentation / MGI
abnormal lipid homeostasis / MGI
no abnormal phenotype detected / MGI
abnormal renal tubule morphology / MGI
dilated renal tubules / MGI
increased liver weight / MGI
maternal imprinting / MGI
yellow coat color / MGI
phenotypic reversion / MGI
maternal effect / MGI
embryonic growth retardation / MGI
abnormal hair follicle melanogenesis / MGI
decreased circulating pituitary hormone level / MGI
abnormal pancreatic islet morphology / MGI
impaired glucose tolerance / MGI
abnormal renal glomerulus morphology / MGI
insulin resistance / MGI
variegated coat color / MGI
endocrine/exocrine gland phenotype / MGI
darkened coat color / MGI
abnormal food intake / MGI
increased susceptibility to weight gain / MGI
increased percent body fat/body weight / MGI
increased circulating leptin level / MGI
abnormal ventral coat pigmentation / MGI
abnormal dorsoventral coat patterning / MGI
abnormal adrenal gland x-zone morphology / MGI
embryonic lethality / MGI
degranulated pancreatic beta cells / MGI
increased gonadal fat pad weight / MGI
increased inguinal fat pad weight / MGI
increased retroperitoneal fat pad weight / MGI
abnormal pinna hair pigmentation / MGI
abnormal tail hair pigmentation / MGI
increased total body fat amount / MGI
mortality/aging / MGI
integument phenotype / MGI
embryonic lethality at implantation, complete penetrance / MGI
embryonic lethality between implantation and placentation, complete penetrance / MGI
expanded mesangial matrix / MGI
renal cast / MGI
increased renal glomerulus basement membrane thickness / MGI
increased food intake / MGI
abnormal epigenetic regulation of gene expression / MGI

Literature references

A new coat color mouse line for testing germline transmission of embryonic stem cells while retaining an inbred genetic background.;Barbaric Ivana, Stewart Michelle, Wells Sara, Dear T Neil, ;2007;Journal of the American Association for Laboratory Animal Science : JAALAS;46;37-40; 17487951

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

129S9.B6-a/H

Information from provider

Information from EMMA

Disease and phenotype information

Literature references

Information on how we integrate external resources can be found here

Availabilities

Practical information

Other EMMA strains