129S9.B6-a/H

Status

Available to order

EMMA IDEM:05339
Citation informationRRID:IMSR_EM:05339 

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International strain name129S9.B6-a/H
Alternative name129S9/SvEvH.B6-a/H
Strain typeSpontaneous
Allele/Transgene symbola
Gene/Transgene symbolA

Information from provider

ProviderSara Wells
Provider affiliationMRC Mammalian Genetics Unit
Genetic informationThe non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a). When chimaeric mice derived from 129S9/SvEvH ES cells are tested for germ line transmission (GLT) it is often performed against a C57BL/6J background to use the non-agouti phenotype as a marker of non-GLT. Mice with Agouti coats are derived from the ES cells and therefore potential carriers of the genetic alteration. These mice however are F1s between C57BL/6 and 129S9/SvEvH. By transferring the a locus to the 129S9/SvEvH background we be able to maintain the 129 genetic background whilst checking for GLT. This strain allows chimaeric mice derived from 129S9/SvEvH ES cells to be tested for germline transmission using coat colour but ensuring that the genetic background is maintained as 129S9/SvEvH.
Phenotypic informationBlack coat colour. The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a).
Breeding historyThe non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a) and maintained by brother x sister mating.
References
  • A new coat color mouse line for testing germline transmission of embryonic stem cells while retaining an inbred genetic background.;Barbaric Ivana, Stewart Michelle, Wells Sara, Dear T Neil, ;2007;Journal of the American Association for Laboratory Animal Science : JAALAS;46;37-40; 17487951
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivinghomozygous males, homozygous females
Breeding at archiving centreThe non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a).
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

MGI phenotypes (gene matching)
  • increased brown adipose tissue amount / MGI
  • irregular coat pigmentation / MGI
  • mottled coat / MGI
  • pigmentation phenotype / MGI
  • increased body length / MGI
  • increased body weight / MGI
  • obese / MGI
  • hypoactivity / MGI
  • abnormal coat appearance / MGI
  • hyperglycemia / MGI
  • increased urine glucose level / MGI
  • neoplasm / MGI
  • increased tumor incidence / MGI
  • decreased incidence of induced tumors / MGI
  • abnormal coat/hair pigmentation / MGI
  • increased circulating insulin level / MGI
  • prenatal lethality / MGI
  • abnormal skin pigmentation / MGI
  • abnormal lipid homeostasis / MGI
  • no abnormal phenotype detected / MGI
  • abnormal renal tubule morphology / MGI
  • dilated renal tubules / MGI
  • increased liver weight / MGI
  • maternal imprinting / MGI
  • yellow coat color / MGI
  • phenotypic reversion / MGI
  • maternal effect / MGI
  • embryonic growth retardation / MGI
  • abnormal hair follicle melanogenesis / MGI
  • decreased circulating pituitary hormone level / MGI
  • abnormal pancreatic islet morphology / MGI
  • impaired glucose tolerance / MGI
  • abnormal renal glomerulus morphology / MGI
  • insulin resistance / MGI
  • variegated coat color / MGI
  • endocrine/exocrine gland phenotype / MGI
  • darkened coat color / MGI
  • abnormal food intake / MGI
  • increased susceptibility to weight gain / MGI
  • increased percent body fat/body weight / MGI
  • increased circulating leptin level / MGI
  • abnormal ventral coat pigmentation / MGI
  • abnormal dorsoventral coat patterning / MGI
  • abnormal adrenal gland x-zone morphology / MGI
  • embryonic lethality / MGI
  • degranulated pancreatic beta cells / MGI
  • increased gonadal fat pad weight / MGI
  • increased inguinal fat pad weight / MGI
  • increased retroperitoneal fat pad weight / MGI
  • abnormal pinna hair pigmentation / MGI
  • abnormal tail hair pigmentation / MGI
  • increased total body fat amount / MGI
  • mortality/aging / MGI
  • integument phenotype / MGI
  • embryonic lethality at implantation, complete penetrance / MGI
  • embryonic lethality between implantation and placentation, complete penetrance / MGI
  • expanded mesangial matrix / MGI
  • renal cast / MGI
  • increased renal glomerulus basement membrane thickness / MGI
  • increased food intake / MGI
  • abnormal epigenetic regulation of gene expression / MGI

Literature references

  • A new coat color mouse line for testing germline transmission of embryonic stem cells while retaining an inbred genetic background.;Barbaric Ivana, Stewart Michelle, Wells Sara, Dear T Neil, ;2007;Journal of the American Association for Laboratory Animal Science : JAALAS;46;37-40; 17487951

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
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