MGI phenotypes (allele matching)
B6N.Cg-Mirc12tm1.1Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:05331 |
| Citation information | RRID:IMSR_EM:05331 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N.Cg-Mirc12tm1.1Doca/Cnrm |
| Alternative name | MiR-144/451 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Mirc12tm1.1Doca |
| Gene/Transgene symbol | Mirc12 |
Information from provider
| Provider | Dónal O |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | An enhanced GFP (eGFP) expression cassette with a 3'- FRT flanked neor cassette was inserted into the miR-144/451 locus. Flp mediated recombination removed the neor cassette. The presence of the eGFP cassette disrupts expression of miR-144 and miR-451 and allows tracing of transcriptional activity from the miR-144/451 locus. |
| Phenotypic information | Homozygous mice carrying the miR-144/451-EGFP-KI allele display an impairment of late erythroblast maturation, resulting in erythroid hyperplasia, splenomegaly, and a mild anemia. |
| Breeding history | Upon germ line transmission, the mice carrying the targeted allele were crossed to FLP-expressing transgenic mice (FLPeR) to remove the frt flanked neor cassette. The resulting mice were then backcrossed to C57BL/6N to remove the FLP transgene. The offspring carrying the mutation were backcrossed 5 generations to C57BL/6N. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- The miR-144/451 locus is required for erythroid homeostasis.;Rasmussen Kasper D, Simmini Salvatore, Abreu-Goodger Cei, Bartonicek Nenad, Di Giacomo Monica, Bilbao-Cortes Daniel, Horos Rastislav, Von Lindern Marieke, Enright Anton J, O'Carroll Dónal, ;2010;The Journal of experimental medicine;207;1351-8; 20513743