B6N.129P2(Cg)-Mir451atm1.1Doca/Cnrm

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EMMA IDEM:05329
Citation informationRRID:IMSR_EM:05329 

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International strain nameB6N.129P2(Cg)-Mir451atm1.1Doca/Cnrm
Alternative nameMir451
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolMir451atm1.1Doca
Gene/Transgene symbolMir451a

Information from provider

ProviderDónal O
Provider affiliationMouse Biology Unit, European Molecular Biology Laboratory
Genetic informationAn FRT flanked neo cassette with a 5' loxP site was inserted downstream of the miR-144/451 locus. An additional loxP site was inserted between the two microRNAs. Flp mediated recombination removed the neo cassette leaving miR-451 floxed. The miR-451 targeting strategy allows cre-mediated deletion of miR-451 within the miR-144/451 cluster, leaving expression of miR-144 intact.
Phenotypic informationMice deficient for the miR-451 display a cell autonomous impairment of late erythroblast maturation, resulting in erythroid hyperplasia, splenomegaly, and a mild anemia.
Breeding historyUpon germ line transmission, the mice carrying the targeted allele were crossed to FLP-expressing transgenic mice (FLPeR) to remove the frt flanked neo-resistance cassette. The resulting mice were backcrossed to C57BL/6N to remove the FLP transgene. The offspring carrying the loxP-flanked allele were backcrossed 6 generations to C57BL/6N.
References
  • The miR-144/451 locus is required for erythroid homeostasis.;Rasmussen Kasper D, Simmini Salvatore, Abreu-Goodger Cei, Bartonicek Nenad, Di Giacomo Monica, Bilbao-Cortes Daniel, Horos Rastislav, Von Lindern Marieke, Enright Anton J, O'Carroll Dónal, ;2010;The Journal of experimental medicine;207;1351-8; 20513743
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (gene matching)
  • decreased hematocrit / MGI
  • abnormal erythropoiesis / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • anemia / MGI
  • abnormal erythrocyte cell number / MGI
  • abnormal hematopoietic system morphology/development / MGI
  • decreased mean corpuscular volume / MGI
  • reticulocytosis / MGI
  • anisocytosis / MGI
  • decreased hemoglobin content / MGI
  • decreased erythrocyte cell number / MGI
  • no phenotypic analysis / MGI
  • increased erythroid progenitor cell number / MGI
  • increased spleen weight / MGI
  • polychromatophilia / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • increased cellular sensitivity to hydrogen peroxide / MGI
  • abnormal physiological response to xenobiotic / MGI
  • increased physiological sensitivity to xenobiotic / MGI
  • increased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • increased red blood cell distribution width / MGI
  • increased number of Heinz bodies / MGI
  • abnormal fetal derived definitive erythrocyte cell number / MGI
  • decreased catalase activity / MGI

Literature references

  • The miR-144/451 locus is required for erythroid homeostasis.;Rasmussen Kasper D, Simmini Salvatore, Abreu-Goodger Cei, Bartonicek Nenad, Di Giacomo Monica, Bilbao-Cortes Daniel, Horos Rastislav, Von Lindern Marieke, Enright Anton J, O'Carroll Dónal, ;2010;The Journal of experimental medicine;207;1351-8; 20513743

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