B6.Cg-Tg(TNF)197Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:05326 |
| International strain name | B6.Cg-Tg(TNF)197Gkl/Flmg |
| Alternative name | B6.Cg-Tg(TNF-HBB)197Gkl/Flmg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(TNF)197Gkl |
| Gene/Transgene symbol | Tg(TNF)197Gkl |
Information from provider
| Provider | George Kollias |
| Provider affiliation | B.S.R.C. |
| Genetic information | The human TNF/beta-globin (TNF-globin) recombinant gene construct was prepared in the pBluescript vector as follows: The 2.8 kb EcoRI fragment which contains the entire coding region of the human TNF gene was ligated to the 0.77 kb EcoRI-Sall fragment which contains the 3'-untranslated region and polyadenylation site of the human beta-globin gene (Kollias et al., 1987) and excised for microinjection as a BamHI-Sall fragment. |
| Phenotypic information | Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis. |
| Breeding history | Backcrossed to C57BL/6J for more than 10 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous 0, wild-type 0 |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis.;Keffer J, Probert L, Cazlaris H, Georgopoulos S, Kaslaris E, Kioussis D, Kollias G, ;1991;The EMBO journal;10;4025-31; 1721867