C57BL/6NTac-Atpif1tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05233
International strain nameC57BL/6NTac-Atpif1tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0158_4_B04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAtpif1tm1a(EUCOMM)Wtsi
Gene/Transgene symbolAtp5if1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0158_4_B04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased body weight / IMPC
MGI phenotypes (allele matching)
  • decreased body weight / MGI
  • decreased circulating alanine transaminase level / MGI
  • decreased circulating alkaline phosphatase level / MGI
  • decreased total body fat amount / MGI
MGI phenotypes (gene matching)
  • decreased body weight / MGI
  • decreased circulating alanine transaminase level / MGI
  • decreased circulating alkaline phosphatase level / MGI
  • decreased total body fat amount / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • Inhibition of ATPIF1 ameliorates severe mitochondrial respiratory chain dysfunction in mammalian cells.;Chen Walter W, Birsoy Kivanc, Mihaylova Maria M, Snitkin Harriet, Stasinski Iwona, Yucel Burcu, Bayraktar Erol C, Carette Jan E, Clish Clary B, Brummelkamp Thijn R, Sabatini David D, Sabatini David M, ;2014;Cell reports;7;27-34; 24685140
  • The ATPase Inhibitory Factor 1 (IF1): A master regulator of energy metabolism and of cell survival.;García-Bermúdez Javier, Cuezva José M, ;2016;Biochimica et biophysica acta;1857;1167-1182; 26876430
  • Upregulation of mitochondrial ATPase inhibitory factor 1 (ATPIF1) mediates increased glycolysis in mouse hearts.;Zhou Bo, Caudal Arianne, Tang Xiaoting, Chavez Juan D, McMillen Timothy S, Keller Andrew, Villet Outi, Zhao Mingyue, Liu Yaxin, Ritterhoff Julia, Wang Pei, Kolwicz Stephen C, Wang Wang, Bruce James E, Tian Rong, ;2022;The Journal of clinical investigation;132;zqac001; 35575090
  • ATP Synthase K+- and H+-fluxes Drive ATP Synthesis and Enable Mitochondrial K+-"Uniporter" Function: II. Ion and ATP Synthase Flux Regulation.;Juhaszova Magdalena, Kobrinsky Evgeny, Zorov Dmitry B, Nuss H Bradley, Yaniv Yael, Fishbein Kenneth W, de Cabo Rafael, Montoliu Lluis, Gabelli Sandra B, Aon Miguel A, Cortassa Sonia, Sollott Steven J, ;2022;Function (Oxford, England);3;e3001252; 35187492
  • Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition.;Esparza-Moltó Pau B, Romero-Carramiñana Inés, Núñez de Arenas Cristina, Pereira Marta P, Blanco Noelia, Pardo Beatriz, Bates Georgina R, Sánchez-Castillo Carla, Artuch Rafael, Murphy Michael P, Esteban José A, Cuezva José M, ;2021;PLoS biology;19;zqab065; 33983919
  • ATP Synthase K+- and H+-Fluxes Drive ATP Synthesis and Enable Mitochondrial K+-"Uniporter" Function: I. Characterization of Ion Fluxes.;Juhaszova Magdalena, Kobrinsky Evgeny, Zorov Dmitry B, Nuss H Bradley, Yaniv Yael, Fishbein Kenneth W, de Cabo Rafael, Montoliu Lluis, Gabelli Sandra B, Aon Miguel A, Cortassa Sonia, Sollott Steven J, ;2022;Function (Oxford, England);3;4870-4891; 35229078
  • IF1 is a cold-regulated switch of ATP synthase hydrolytic activity to support thermogenesis in brown fat.;Brunetta Henver S, Jung Anna S, Valdivieso-Rivera Fernando, de Campos Zani Stepheny C, Guerra Joel, Furino Vanessa O, Francisco Annelise, Berçot Marcelo, Moraes-Vieira Pedro M, Keipert Susanne, Jastroch Martin, Martinez Laurent O, Sponton Carlos H, Castilho Roger F, Mori Marcelo A, Bartelt Alexander, ;2024;The EMBO journal;43;; 39284909

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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