B6Brd;B6N-Tyrc-Brd Ncaph2tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05225
Citation informationRRID:IMSR_EM:05225 

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International strain nameB6Brd;B6N-Tyrc-Brd Ncaph2tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0070_2_G09
Strain typeTargeted Mutant Strains
Allele/Transgene symbolNcaph2tm1a(EUCOMM)Wtsi
Gene/Transgene symbolNcaph2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0070_2_G09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal glucose homeostasis / IMPC
  • increased circulating amylase level / IMPC
  • preweaning lethality, complete penetrance / IMPC
IMPC phenotypes (gene matching)
  • abnormal glucose homeostasis / IMPC
  • increased circulating amylase level / IMPC
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (allele matching)
  • increased circulating amylase level / MGI
MGI phenotypes (gene matching)
  • microcephaly / MGI
  • decreased thymocyte number / MGI
  • abnormal cerebral cortex morphology / MGI
  • decreased body weight / MGI
  • thymus hypoplasia / MGI
  • decreased brain weight / MGI
  • abnormal thymus cortex morphology / MGI
  • abnormal double-negative T cell morphology / MGI
  • abnormal mitosis / MGI
  • decreased T cell number / MGI
  • decreased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • abnormal T cell morphology / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • increased circulating amylase level / MGI
  • abnormal splenic cell ratio / MGI
  • abnormal thymus cell ratio / MGI
  • abnormal thymus corticomedullary boundary morphology / MGI
  • embryonic lethality, complete penetrance / MGI
  • abnormal neocortex size / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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