C57BL/6N-Sirt6^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:05219
International strain name	C57BL/6N-Sirt6^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0256_5_F08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Sirt6^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Sirt6
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0256_5_F08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac
Breeding at archiving centre	Chimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Lethal brain and heart developmental defects / Orphanet_580933

IMPC phenotypes (allele matching)

increased startle reflex / IMPC
increased circulating cholesterol level / IMPC
increased mean corpuscular hemoglobin concentration / IMPC

IMPC phenotypes (gene matching)

increased mean corpuscular hemoglobin concentration / IMPC
increased circulating cholesterol level / IMPC
increased startle reflex / IMPC

MGI phenotypes (gene matching)

decreased bone mineral density / MGI
malocclusion / MGI
kyphosis / MGI
lordosis / MGI
hypoglycemia / MGI
decreased leukocyte cell number / MGI
decreased bone marrow cell number / MGI
decreased cell proliferation / MGI
spleen hypoplasia / MGI
decreased body length / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal nursing / MGI
postnatal growth retardation / MGI
reduced female fertility / MGI
male infertility / MGI
abnormal glucose homeostasis / MGI
premature death / MGI
abnormal eye morphology / MGI
abnormal B cell differentiation / MGI
abnormal lymphopoiesis / MGI
decreased circulating insulin level / MGI
colitis / MGI
increased insulin sensitivity / MGI
premature aging / MGI
spontaneous chromosome breakage / MGI
increased cellular sensitivity to ionizing radiation / MGI
decreased circulating insulin-like growth factor I level / MGI
decreased double-positive T cell number / MGI
narrow eye opening / MGI
decreased circulating glucose level / MGI
early cellular replicative senescence / MGI
increased cellular sensitivity to alkylating agents / MGI
abnormal spleen physiology / MGI
decreased subcutaneous adipose tissue amount / MGI
decreased total body fat amount / MGI
lethality at weaning, complete penetrance / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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