C57BL/6NTac-Abcb11^{tm1a(EUCOMM)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:05200
International strain name	C57BL/6NTac-Abcb11^{tm1a(EUCOMM)Hmgu}/Ieg
Alternative name	HEPD0507_7_B05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Abcb11^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Abcb11
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0507_7_B05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTacUSA, wild-type C57BL/6NTacUSA
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Progressive familial intrahepatic cholestasis type 2 / Orphanet_79304
Benign recurrent intrahepatic cholestasis type 2 / Orphanet_99961

IMPC phenotypes (allele matching)

abnormal retina morphology / IMPC
increased circulating alkaline phosphatase level / IMPC
increased lean body mass / IMPC
increased circulating potassium level / IMPC

IMPC phenotypes (gene matching)

increased circulating amylase level / IMPC
abnormal retina morphology / IMPC
increased circulating aspartate transaminase level / IMPC
increased circulating alkaline phosphatase level / IMPC
increased circulating potassium level / IMPC
abnormal lung morphology / IMPC
increased circulating unsaturated transferrin level / IMPC
decreased locomotor activity / IMPC
decreased circulating triglyceride level / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased circulating fructosamine level / IMPC
increased lean body mass / IMPC
increased circulating bilirubin level / IMPC

MGI phenotypes (gene matching)

enlarged liver / MGI
abnormal hepatocyte morphology / MGI
decreased body weight / MGI
postnatal growth retardation / MGI
increased cholesterol level / MGI
abnormal bile secretion / MGI
abnormal bile salt level / MGI
abnormal phospholipid level / MGI
increased bile salt level / MGI
intrahepatic cholestasis / MGI
decreased glycogen level / MGI
abnormal bile canaliculus morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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