NOD.FVB-Tg(Rip-RASA1*)1Wid/Orl
| Status | Available to order |
| EMMA ID | EM:05139 |
| Citation information | RRID:IMSR_EM:05139 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | NOD.FVB-Tg(Rip-RASA1*)1Wid/Orl |
| Alternative name | NOD-Tg(RIP::N)1Wid |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Rip-RASA1*)1Wid |
| Gene/Transgene symbol | Tg(Rip-RASA1*)1Wid |
Information from provider
| Provider | Christian Widmann |
| Provider affiliation | Physiology, Lausanne University |
| Genetic information | NOD-Tg(Rip-RASA1*)1Wid (TgN(RIP::N)1Wid) mice bear a transgene containing the rat insulin promoter followed by the cDNA encoding a caspase-resistant form of N-terminal fragment of human RasGAP. |
| Phenotypic information | The NOD background induces auto-immune type 1 diabetes with a 60-80% and 20-30% penetrance in females and males, respectively. The presence of fragment N in pancreatic beta cells of NOD mice increases their resistance towards apoptosis and delays the onset of overt diabetes. |
| Breeding history | The RIP-N transgene from FVB/N-Tg(RIP::N)1Wid mice (strain EM:04569) was backcrossed into NOD mice using speed-congenics (first 5 generations) and conventional backcrossing for 2 subsequent generations. The strain is maintained in the NOD background (currently 2 additional generations). |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous NOD males, wild-type NOD females |
Literature references
- Expression of the NH(2)-terminal fragment of RasGAP in pancreatic beta-cells increases their resistance to stresses and protects mice from diabetes.;Yang Jiang-Yan, Walicki Jöel, Jaccard Evrim, Dubuis Gilles, Bulat Natasa, Hornung Jean-Pierre, Thorens Bernard, Widmann Christian, ;2009;Diabetes;58;2596-606; 19696184
- Role of mTOR, Bad, and Survivin in RasGAP Fragment N-Mediated Cell Protection.;Peltzer Nieves, Vanli Güliz, Yang Jiang-Yan, Widmann Christian, ;2013;PloS one;8;e68123; 23826368