C57BL/6N-Acp2^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:05128
Citation information	RRID:IMSR_EM:05128 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Acp2^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0370_6_H05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Acp2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Acp2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0370_6_H05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac males
Breeding at archiving centre	Chimeras were mated to C57BL/6N Tac USA and maintained on this background thereafter.

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
abnormal bone mineralization / IMPC
abnormal bone structure / IMPC
abnormal behavior / IMPC
increased B cell number / IMPC
decreased T cell number / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
increased bone mineral content / IMPC

IMPC phenotypes (gene matching)

increased B cell number / IMPC
decreased erythrocyte cell number / IMPC
decreased hemoglobin content / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
abnormal bone structure / IMPC
abnormal bone mineralization / IMPC
decreased bone mineral density / IMPC
increased bone mineral content / IMPC
abnormal behavior / IMPC

MGI phenotypes (gene matching)

kyphoscoliosis / MGI
abnormal microglial cell morphology / MGI
abnormal vertebral body morphology / MGI
kyphosis / MGI
lordosis / MGI
abnormal hair follicle morphology / MGI
alopecia / MGI
delayed hair appearance / MGI
tremors / MGI
abnormal cerebellum morphology / MGI
small cerebellum / MGI
thin external granule cell layer / MGI
abnormal cerebellar Purkinje cell layer / MGI
ectopic Purkinje cell / MGI
absent cerebellar granule layer / MGI
abnormal cerebellar molecular layer / MGI
abnormal CNS glial cell morphology / MGI
abnormal epidermis stratum basale morphology / MGI
abnormal dermal layer morphology / MGI
decreased body height / MGI
decreased body weight / MGI
ataxia / MGI
postnatal growth retardation / MGI
astrocytosis / MGI
abnormal compact bone morphology / MGI
tonic-clonic seizures / MGI
abnormal vertebral pedicle morphology / MGI
abnormal vertebral column morphology / MGI
abnormal lysosome morphology / MGI
accumulation of giant lysosomes in kidney/renal tubule cells / MGI
behavior/neurological phenotype / MGI
abnormal Purkinje cell dendrite morphology / MGI
thin hair shaft / MGI
increased trabecular bone connectivity density / MGI
decreased bone mineralization / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Acp2tm1a(EUCOMM)Wtsi/H