C57BL/6N-P2rx7^{tm1a(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:05116
Citation information	RRID:IMSR_EM:05116 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-P2rx7^{tm1a(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0369_6_A06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	P2rx7^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	P2rx7
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0369_6_A06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	The eATP/P2×7R Axis Drives Quantum Dot-Nanoparticle Induced Neutrophil Recruitment in the Pulmonary Microcirculation.;Li Chenxi, Liu Qiongliang, Han Lianyong, Zhang Haiyun, Immler Roland, Rathkolb Birgit, Secklehner Judith, de Angelis Martin Hrabe, Yildirim Ali Önder, Zeuschner Dagmar, Nicke Annette, Carlin Leo M, Sperandio Markus, Stoeger Tobias, Rehberg Markus, ;2024;Advanced science (Weinheim, Baden-Wurttemberg, Germany);11;e2404661; 39364760

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac (USA) males

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased circulating glycerol level / IMPC

IMPC phenotypes (gene matching)

cataract / IMPC
decreased circulating glycerol level / IMPC
abnormal lens morphology / IMPC

MGI phenotypes (gene matching)

abnormal cell death / MGI
decreased body weight / MGI
abnormal skeleton physiology / MGI
abnormal apoptosis / MGI
abnormal inflammatory response / MGI
abnormal pain threshold / MGI
abnormal skeleton development / MGI
abnormal mast cell physiology / MGI
abnormal macrophage physiology / MGI
increased coping response / MGI
increased osteoclast cell number / MGI
hearing/vestibular/ear phenotype / MGI
cardiovascular system phenotype / MGI
behavior/neurological phenotype / MGI
immune system phenotype / MGI
decreased diameter of long bones / MGI
decreased diameter of femur / MGI
decreased diameter of tibia / MGI
decreased interleukin-1 beta secretion / MGI
abnormal interleukin-6 secretion / MGI
increased compensatory feeding amount / MGI

Literature references

The eATP/P2×7R Axis Drives Quantum Dot-Nanoparticle Induced Neutrophil Recruitment in the Pulmonary Microcirculation.;Li Chenxi, Liu Qiongliang, Han Lianyong, Zhang Haiyun, Immler Roland, Rathkolb Birgit, Secklehner Judith, de Angelis Martin Hrabe, Yildirim Ali Önder, Zeuschner Dagmar, Nicke Annette, Carlin Leo M, Sperandio Markus, Stoeger Tobias, Rehberg Markus, ;2024;Advanced science (Weinheim, Baden-Wurttemberg, Germany);11;e2404661; 39364760
Extracellular ATP protects against sepsis through macrophage P2X7 purinergic receptors by enhancing intracellular bacterial killing.;Csóka Balázs, Németh Zoltán H, Törő Gábor, Idzko Marco, Zech Andreas, Koscsó Balázs, Spolarics Zoltán, Antonioli Luca, Cseri Karolina, Erdélyi Katalin, Pacher Pál, Haskó György, ;2015;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;29;3626-37; 26060214
P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus.;Faliti Caterina E, Gualtierotti Roberta, Rottoli Elsa, Gerosa Maria, Perruzza Lisa, Romagnani Andrea, Pellegrini Giovanni, De Ponte Conti Benedetta, Rossi Riccardo L, Idzko Marco, Mazza Emilia M C, Bicciato Silvio, Traggiai Elisabetta, Meroni Pier Luigi, Grassi Fabio, ;2019;The Journal of experimental medicine;216;317-336; 30655308
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.;Kaczmarek-Hajek Karina, Zhang Jiong, Kopp Robin, Grosche Antje, Rissiek Björn, Saul Anika, Bruzzone Santina, Engel Tobias, Jooss Tina, Krautloher Anna, Schuster Stefanie, Magnus Tim, Stadelmann Christine, Sirko Swetlana, Koch-Nolte Friedrich, Eulenburg Volker, Nicke Annette, ;2018;eLife;7;; 30074479

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-P2rx7tm1a(EUCOMM)Wtsi/Ieg