B6Brd;B6N-Tyr^c-Brd Fto^{tm1a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:05094
International strain name	B6Brd;B6N-Tyr^c-Brd Fto^{tm1a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0103_5_A10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Fto^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Fto
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0103_5_A10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Lethal polymalformative syndrome, Boissel type / Orphanet_210144

IMPC phenotypes (allele matching)

decreased circulating alanine transaminase level / IMPC
decreased circulating iron level / IMPC
decreased circulating cholesterol level / IMPC

IMPC phenotypes (gene matching)

decreased circulating cholesterol level / IMPC
decreased circulating iron level / IMPC
decreased circulating alanine transaminase level / IMPC

MGI phenotypes (allele matching)

decreased circulating LDL cholesterol level / MGI
hyperactivity / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating iron level / MGI
decreased lactate dehydrogenase level / MGI
decreased circulating aspartate transaminase level / MGI

MGI phenotypes (gene matching)

decreased bone mineral density / MGI
decreased circulating LDL cholesterol level / MGI
dermatitis / MGI
decreased body length / MGI
decreased body weight / MGI
hyperactivity / MGI
hypoactivity / MGI
abnormal eating behavior / MGI
polyphagia / MGI
increased circulating triglyceride level / MGI
increased circulating HDL cholesterol level / MGI
postnatal growth retardation / MGI
increased circulating glucagon level / MGI
decreased circulating insulin level / MGI
decreased circulating alanine transaminase level / MGI
increased lean body mass / MGI
decreased lean body mass / MGI
decreased circulating iron level / MGI
decreased circulating insulin-like growth factor I level / MGI
increased energy expenditure / MGI
cachexia / MGI
increased circulating cholesterol level / MGI
abnormal metabolism / MGI
abnormal oxygen consumption / MGI
increased oxygen consumption / MGI
improved glucose tolerance / MGI
homeostasis/metabolism phenotype / MGI
growth/size/body region phenotype / MGI
abnormal circulating hormone level / MGI
decreased percent body fat/body weight / MGI
increased circulating glucose level / MGI
decreased lactate dehydrogenase level / MGI
decreased circulating aspartate transaminase level / MGI
increased susceptibility to diet-induced obesity / MGI
decreased susceptibility to diet-induced obesity / MGI
increased circulating adrenaline level / MGI
decreased circulating leptin level / MGI
increased circulating leptin level / MGI
abnormal carbon dioxide production / MGI
increased carbon dioxide production / MGI
decreased carbon dioxide production / MGI
increased basal metabolism / MGI
decreased white fat cell size / MGI
decreased gonadal fat pad weight / MGI
increased total body fat amount / MGI
decreased total body fat amount / MGI
increased respiratory quotient / MGI
decreased respiratory quotient / MGI
postnatal lethality, incomplete penetrance / MGI
preweaning lethality, incomplete penetrance / MGI
abnormal urine catecholamine level / MGI
increased food intake / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
The Role of m⁶A/m-RNA Methylation in Stress Response Regulation.;Engel Mareen, Eggert Carola, Kaplick Paul M, Eder Matthias, Röh Simone, Tietze Lisa, Namendorf Christian, Arloth Janine, Weber Peter, Rex-Haffner Monika, Geula Shay, Jakovcevski Mira, Hanna Jacob H, Leshkowitz Dena, Uhr Manfred, Wotjak Carsten T, Schmidt Mathias V, Deussing Jan M, Binder Elisabeth B, Chen Alon, ;2018;Neuron;99;389-403.e9; 30048615

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Ftotm1a(EUCOMM)Wtsi/WtsiCnbc