B6Brd;B6N-Tyr^c-Brd Slx4^{tm1a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:05091
International strain name	B6Brd;B6N-Tyr^c-Brd Slx4^{tm1a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0028_7_A08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Slx4^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Slx4
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0028_7_A08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Fanconi anemia / Orphanet_84

IMPC phenotypes (allele matching)

preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC

MGI phenotypes (allele matching)

decreased leukocyte cell number / MGI
abnormal cranium morphology / MGI
enlarged cranium / MGI
domed cranium / MGI
abnormal cerebral cortex morphology / MGI
dilated lateral ventricles / MGI
abnormal third ventricle morphology / MGI
obstructive hydrocephaly / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal spermatogenesis / MGI
decreased body weight / MGI
decreased body size / MGI
anophthalmia / MGI
microphthalmia / MGI
abnormal cornea morphology / MGI
corneal opacity / MGI
abnormal pupil morphology / MGI
abnormal iris morphology / MGI
absent optic nerve / MGI
postnatal growth retardation / MGI
hydroencephaly / MGI
reduced male fertility / MGI
reduced female fertility / MGI
decreased litter size / MGI
abnormal eye morphology / MGI
abnormal tooth morphology / MGI
abnormal lymphopoiesis / MGI
oligozoospermia / MGI
abnormal retinal vasculature morphology / MGI
increased erythrocyte cell number / MGI
thrombocytopenia / MGI
nervous system phenotype / MGI
abnormal eyelid aperture / MGI
abnormal spine curvature / MGI
vertebral fusion / MGI
absent oocytes / MGI
azoospermia / MGI
impaired glucose tolerance / MGI
increased circulating glucose level / MGI
thin cerebral cortex / MGI
early cellular replicative senescence / MGI
abnormal male germ cell apoptosis / MGI
abnormal primordial germ cell morphology / MGI
decreased common myeloid progenitor cell number / MGI
chromosomal instability / MGI
increased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced cell death / MGI
increased caudal vertebrae number / MGI
perinatal lethality, incomplete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
abnormal eye size / MGI

MGI phenotypes (gene matching)

decreased leukocyte cell number / MGI
abnormal cranium morphology / MGI
enlarged cranium / MGI
domed cranium / MGI
abnormal cerebral cortex morphology / MGI
dilated lateral ventricles / MGI
abnormal third ventricle morphology / MGI
obstructive hydrocephaly / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
small testis / MGI
abnormal spermatogenesis / MGI
decreased body weight / MGI
decreased body size / MGI
anophthalmia / MGI
microphthalmia / MGI
abnormal cornea morphology / MGI
corneal opacity / MGI
abnormal pupil morphology / MGI
abnormal iris morphology / MGI
absent optic nerve / MGI
postnatal growth retardation / MGI
hydroencephaly / MGI
reduced male fertility / MGI
reduced female fertility / MGI
decreased litter size / MGI
abnormal eye morphology / MGI
abnormal tooth morphology / MGI
abnormal lymphopoiesis / MGI
oligozoospermia / MGI
abnormal eye size / MGI
abnormal retinal vasculature morphology / MGI
no phenotypic analysis / MGI
increased erythrocyte cell number / MGI
thrombocytopenia / MGI
nervous system phenotype / MGI
abnormal eyelid aperture / MGI
induced chromosome breakage / MGI
abnormal spine curvature / MGI
vertebral fusion / MGI
absent oocytes / MGI
azoospermia / MGI
impaired glucose tolerance / MGI
increased circulating glucose level / MGI
thin cerebral cortex / MGI
early cellular replicative senescence / MGI
abnormal DNA repair / MGI
abnormal male germ cell apoptosis / MGI
abnormal primordial germ cell morphology / MGI
decreased common myeloid progenitor cell number / MGI
chromosomal instability / MGI
increased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced cell death / MGI
increased caudal vertebrae number / MGI
abnormal telomere morphology / MGI
perinatal lethality, incomplete penetrance / MGI
preweaning lethality, complete penetrance / MGI
embryonic lethality, incomplete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
preweaning lethality, incomplete penetrance / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Slx4tm1a(EUCOMM)Wtsi/WtsiCnbc