B6Dnk;B6Brd;B6N-Tyrc-Brd Rad18tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05089
International strain nameB6Dnk;B6Brd;B6N-Tyrc-Brd Rad18tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0027_4_B02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolRad18tm1a(EUCOMM)Wtsi
Gene/Transgene symbolRad18
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0027_4_B02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal coat/ hair morphology / IMPC
  • decreased body weight / IMPC
  • abnormal lens morphology / IMPC
  • cataract / IMPC
  • increased thermal nociceptive threshold / IMPC
  • abnormal hair texture / IMPC
  • increased mean corpuscular volume / IMPC
  • increased hematocrit / IMPC
  • decreased total body fat amount / IMPC
IMPC phenotypes (gene matching)
  • increased hematocrit / IMPC
  • decreased body weight / IMPC
  • abnormal coat/ hair morphology / IMPC
  • decreased total body fat amount / IMPC
  • increased mean corpuscular volume / IMPC
  • cataract / IMPC
  • increased thermal nociceptive threshold / IMPC
  • abnormal lens morphology / IMPC
  • abnormal hair texture / IMPC
MGI phenotypes (allele matching)
  • abnormal liver physiology / MGI
  • decreased body weight / MGI
  • hyperactivity / MGI
  • increased energy expenditure / MGI
  • increased oxygen consumption / MGI
  • chromosomal instability / MGI
  • increased carbon dioxide production / MGI
  • abnormal coat/ hair morphology / MGI
  • hypoactivity / MGI
  • abnormal hair texture / MGI
  • increased mean corpuscular hemoglobin concentration / MGI
MGI phenotypes (gene matching)
  • abnormal coat/ hair morphology / MGI
  • abnormal liver physiology / MGI
  • seminiferous tubule degeneration / MGI
  • decreased body weight / MGI
  • hyperactivity / MGI
  • hypoactivity / MGI
  • reduced male fertility / MGI
  • abnormal hair texture / MGI
  • decreased testis weight / MGI
  • increased energy expenditure / MGI
  • decreased male germ cell number / MGI
  • increased oxygen consumption / MGI
  • homeostasis/metabolism phenotype / MGI
  • increased mean corpuscular hemoglobin concentration / MGI
  • abnormal spermatogonia morphology / MGI
  • chromosomal instability / MGI
  • increased carbon dioxide production / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).